Gene expression profiling of brain metastatic cell from triple negative breast cancer: Understanding the molecular events

• Published in: Gene Vol. 640; pp. 21 - 27
• Main Authors: Zhou, Li, Gao, Hong-Fang, Liu, Ding-Sheng, Feng, Jin-Yi, Gao, Dan-Dan, Xia, Wei
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.01.2018
• Subjects: Biomarkers, Tumor - genetics; Brain metastasis; Brain Neoplasms - genetics; Brain Neoplasms - secondary; Female; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Gene Ontology; Gene Regulatory Networks; Humans; Microarray analysis; Molecular events; Real-Time Polymerase Chain Reaction; Signal Transduction; Triple negative breast cancer; Triple Negative Breast Neoplasms - genetics; Triple Negative Breast Neoplasms - pathology; Tumor Cells, Cultured; PR; Molecular events; Brain metastasis; GO; PPI; BM; TNBC; HER2; ER; Triple negative breast cancer; Microarray analysis
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/j.gene.2017.10.019

Brain metastatic triple negative breast cancer (BM-TNBC) is afflicted with unfavorable prognosis. However, the molecular events underlying BM-TNBC remain largely unknown. In the present study, we conducted gene expression microarray analysis using the triple negative breast cancer cell line MDA-MB-231 and its brain metastatic derivative (MDA-MB-231Brm). Results of microarray analysis showed that a total of 4296 genes were differentially expressed, of which 2433 genes were up-regulated and 1863 genes were down-regulated. Gene Ontology (GO), KEGG pathway and protein-protein interaction (PPI) analyses indicated differentially expressed genes functionally categorized as genes of signal transduction, multicellular organismal development, ion transport, nervous system development, plasma membrane, extracellular region, calcium ion binding, GTP binding neuroactive ligand-receptor interaction. The validity of the microarray results was verified by quantitative real-time PCR analysis of twelve representative genes. The present findings revealed molecular basis and events associated with brain metastasis in TNBC, which will potentially contribute to the understanding of underlying mechanism and develop therapeutic targets. •The understanding of molecular events underlying TNBC brain metastasis remains poor.•We explored differentially expressed genes using the Agilent SurePrint G3 Human microarray.•GO and KEGG analyses were performed to reveal the molecular events.•PPI network was conducted to identify genes related to nervous system.