Genome-wide identification, phylogenetic analysis, and expression profiling of polyamine synthesis gene family members in tomato

• Published in: Gene Vol. 661; pp. 1 - 10
• Main Authors: Liu, Taibo, Huang, Binbin, Chen, Lin, Xian, Zhiqiang, Song, Shiwei, Chen, Riyuan, Hao, Yanwei
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 30.06.2018
• Subjects: Fruit development; Genomic characterization; Plant stress response; Polyamine synthesis; Solanum lycopersicum; Tomato; ADC; Polyamine synthesis; T-Spm; SPMS; Spd; Solanum lycopersicum; SPDS; Tomato; Put; ODC; PA; Spm; Genomic characterization; ACL5; Plant stress response; Fruit development
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/j.gene.2018.03.084

Polyamines (PAs), including putrescine (Put), spermidine (Spd), spermine (Spm), and thermospermine (T-Spm), play key roles in plant development, including fruit setting and ripening, morphogenesis, and abiotic/biotic stress. Their functions appear to be intimately related to their synthesis, which occurs via arginine/ornithine decarboxylase (ADC/ODC), Spd synthase (SPDS), Spm synthase (SPMS), and Acaulis5 (ACL5), respectively. Unfortunately, the expression and function of these PA synthesis-relate genes during specific developmental process or under stress have not been fully elucidated. Here, we present the results of a genome-wide analysis of the PA synthesis genes (ADC, ODC, SPDS, SPMS, ACL5) in the tomato (Solanum lycopersicum). In total, 14 PA synthesis-related genes were identified. Further analysis of their structures, conserved domains, phylogenetic trees, predicted subcellular localization, and promoter cis-regulatory elements were analyzed. Furthermore, we also performed experiments to evaluate their tissue expression patterns and under hormone and various stress treatments. To our knowledge, this is the first study to elucidate the mechanisms underlying PA function in this variety of tomato. Taken together, these data provide valuable information for future functional characterization of specific genes in the PA synthesis pathway in this and other plant species. Although additional research is required, the insight gained by this and similar studies can be used to improve our understanding of PA metabolism ultimately leading to more effective and consistent plant cultivation. •14 PA synthases genes have been identified in the tomato genome.•PA synthases genes preferentially expressed in vegetative tissues.•Expression level of PA synthases genes were affected by hormone treatment.•Expression level of PA synthases genes were affected by abiotic stress treatment.•SlSPDS2 may play an important role in tomato fruit ripening.