MicroRNAs as serum biomarker for Senecio brasiliensis poisoning in cattle

• Published in: Environmental toxicology and pharmacology Vol. 94; p. 103906
• Main Authors: Winter, Evelyn, Cisilotto, Julia, Goetten, André L.F., Veiga, Ângela, Ramos, Adriano T., Zimermann, Francielli C., Reck, Carolina, Creczynski-Pasa, Tânia B.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.08.2022
• Subjects: Biomarker; Livestock poisoning; MiRNA; Senecio brasiliensis; Biomarker; Livestock poisoning; Senecio brasiliensis; MiRNA; livestock poisoning; miRNA; biomarker
• ISSN: 1382-6689; 1872-7077
• DOI: 10.1016/j.etap.2022.103906

Senecio spp. is one of the most frequent plant-related poisonings in cattle. Its ingestion generates the disease seneciosis, characterized by hepatic damages. Liver biopsies and serum markers dosage are tools used in diagnosis; however, many breeding cattle are undiagnosed. MicroRNAs are non-coding RNA, stable in biological fluids. Their difference in expression levels may indicate the presence of the poisoning. We analyzed the miRNA profiling to identify potential diagnostic biomarkers for Senecio brasiliensis poisoning. The expression of miR-21, miR-885, miR-122, miR-181b, miR-30a, miR-378, and let-7 f were evaluated in the serum of exposed cattle. At least one histological change was found in liver and lower quantity of albumin and high AST and ALP were also detected. MiRNAs miR-30a, miR-378, miR-21, miR-885, and miR-122 presented significantly higher expression in intoxicated animals than in healthy animals. Furthermore, miR-122, miR-885, and, especially, miR-21 signatures demonstrated high sensitivity and specificity, with potential application for detecting poisoning. [Display omitted] •MiR-30a, −378, −21, −885, and −122 were more expressed in intoxicated animals.•MiR-122, −885 and −21 signatures demonstrated high sensitivity and specificity.•MiRNAs can be used as biomarkers for minimally invasive seneciosis diagnostic.