Neodeightonia phoenicum CMIB-151: Isolation, Molecular Identification, and Production and Characterization of an Exopolysaccharide

• Published in: Journal of polymers and the environment Vol. 28; no. 7; pp. 1954 - 1966
• Main Authors: da Silva Fonseca, Michel, Marchioro, Marcelo Luis Kuhn, Guimarães, Denyse K. S., Góes-Neto, Aristóteles, Drechsler-Santos, Elisandro Ricardo, Santos, Vidiany A. Q., Barbosa-Dekker, Aneli M., Dekker, Robert F. H., Cunha, Mário A. A.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.07.2020; Springer Nature B.V
• Subjects: Ammonium; Ammonium sulfate; Carbohydrates; Carbon; Chemistry; Chemistry and Materials Science; Environmental Chemistry; Environmental Engineering/Biotechnology; Exopolysaccharides; Fructose; Fungi; Industrial applications; Industrial Chemistry/Chemical Engineering; Lactose; Maltose; Materials Science; Mycelia; NMR; Nuclear magnetic resonance; Original Paper; Peptones; pH effects; Polymer Sciences; Starch; Sucrose; Sugar; Thermal stability; Urea; Yeasts; Exopolysaccharide; Phylogenetic tree; Ascomycete; Fungal bioprospecting
• ISSN: 1566-2543; 1572-8919
• DOI: 10.1007/s10924-020-01744-5

The ascomycete Neodeightonia phoenicum CMIB-151 was isolated from bracts of a palm tree ( Syagrus romanzoffiana ) in Brazil, and identified by molecular techniques. This is the first report of the isolation of this fungus in South America, and for the first time this fungus has been described as producing an exopolysaccharide (EPS). Different carbon (glucose, fructose, sucrose, maltose, lactose, starch) and nitrogen (yeast extract, peptone, urea, ammonium sulfate) sources were evaluated for the production of EPS. Sucrose and peptone resulted in high production and yield of EPS. High production of EPS occurred at pH 6.0 and 8.0, and acidic conditions (initial pH 5.0) promoted higher mycelial growth. The EPS produced showed a high degree of purity, emulsifying activity and a high water- and oil-holding capacity; important technological properties for industrial applications. FT-IR and 13 C-NMR (CP/MAS) analyzes showed typical spectra of carbohydrates containing six-carbon sugar in its structure, and the presence of β-glycosidic configuration with 3- O -substitution. The isolated EPS exhibited remarkable thermal stability.