Recombinant adeno-associated virus delivered human thioredoxin-PR39 prevents hypoxia-induced apoptosis of ECV304 cells

Human thioredoxin and antibacterial peptide, PR39, have been shown to have potent antioxidant effects that may prolong survival of cells during hypoxia. The pSSCMV/human thioredoxin-PR39 vector was successfully constructed in this study and used to infect ECV304 cells. Transfected ECV304 cells were...

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Published inNeural regeneration research Vol. 7; no. 9; pp. 708 - 713
Main Authors Ruan, Xiyun, Yuan, Zhenguo, Du, Yifeng, Yang, Guangxiao, Wang, Quanying
Format Journal Article
LanguageEnglish
Published India Medknow Publications & Media Pvt. Ltd 25.03.2012
Department of Neurology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong Province, China%Shandong Medical Imaging Research Institute, Affiliated to Shandong University, Jinan 250021, Shandong Province, China%Xi'an Huaguang Biological Engineering Co., Ltd., Xi'an 710025, Shaanxi Province, China
Medknow Publications & Media Pvt Ltd
Subjects
Adenoviruses
Apoptosis
Bioengineering
Blood-brain barrier
Deoxyribonucleic acid
DNA
E coli
Engineering
Gene expression
Hypoxia
Microscopy
Morphology
Nervous system
Peptides
Plasmids
Proteins
Techniques and Method: Emerging Technology in Neuroregeneration
Vectors (Biology)
交付
人硫氧还蛋白
抗氧化作用
细胞凋亡
缺氧条件
血管内皮
诱导
重组腺相关病毒
China
human thioredoxin
hypoxia
recombinant adeno-associated virus
fusion gene
apoptosis
gene therapy
antimicrobial peptide PR39
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Summary:Human thioredoxin and antibacterial peptide, PR39, have been shown to have potent antioxidant effects that may prolong survival of cells during hypoxia. The pSSCMV/human thioredoxin-PR39 vector was successfully constructed in this study and used to infect ECV304 cells. Transfected ECV304 cells were incubated at 1%, 5% hypoxic, and normal oxygen conditions. We found that the number of apoptotic cells after transfection with recombinant adeno-associated virus-human thioredoxin -PR39 was significantly lower than controls, suggesting a protective effect of the recombinant human thioredoxin-PR39 protein on hypoxic cells.
Bibliography:Human thioredoxin and antibacterial peptide, PR39, have been shown to have potent antioxidant effects that may prolong survival of cells during hypoxia. The pSSCMV/human thioredoxin-PR39 vector was successfully constructed in this study and used to infect ECV304 cells. Transfected ECV304 cells were incubated at 1%, 5% hypoxic, and normal oxygen conditions. We found that the number of apoptotic cells after transfection with recombinant adeno-associated virus-human thioredoxin -PR39 was significantly lower than controls, suggesting a protective effect of the recombinant human thioredoxin-PR39 protein on hypoxic cells.
human thioredoxin antimicrobial peptide PR39 fusion gene recombinant adeno-associated virus gene therapy apoptosis hypoxia
11-5422/R
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Author contributions: Guangxiao Yang, Quanying Wang and Yifeng Du had full access to the study concept and design, and revised the manuscript. Xiyun Ruan wrote the manuscript and was responsible for funding. Zhenguo Yuan analyzed data and participated in statistical analyses.
Xiyun Ruan, M.D., Associate chief physician, Department of Neurology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong Province, China
ISSN:1673-5374
1876-7958
DOI:10.3969/j.issn.1673-5374.2012.09.012