Highly emissive deoxyguanosine analogue capable of direct visualization of B-Z transition

• Published in: Chemical communications (Cambridge, England) Vol. 50; no. 13; pp. 1573 - 1575
• Main Authors: Park, Soyoung, Otomo, Haruka, Zheng, Linjie, Sugiyama, Hiroshi
• Format: Journal Article
• Language: English
• Published: CAMBRIDGE Royal Soc Chemistry 01.01.2014
• Subjects: Analogue; Base Sequence; Chemistry; Chemistry, Multidisciplinary; Deoxyguanosine - analogs & derivatives; DNA, B-Form - analysis; DNA, Z-Form - analysis; Emissivity; Fluorescent Dyes - chemistry; Guanines; Oligonucleotides; Oligonucleotides - chemistry; Physical Sciences; Science & Technology; Visual; Visualization; HANDED Z-DNA; NUCLEOTIDES; BASE-PAIR; 2-AMINOPURINE FLUORESCENCE; QUADRUPLEX; RNA; NUCLEOSIDES; POLYMERASE; DERIVATIVES; PROBES

A 2-aminothieno[3,4-d] pyrimidine G-mimic deoxyribonucleoside, thdG, was synthesized and incorporated readily into oligonucleotides as a versatile fluorescent guanine analogue. We demonstrate that thdG enables the visual detection of Z-DNA successfully based on different pi-stacking of B- and Z-DNA.