Highly emissive deoxyguanosine analogue capable of direct visualization of B-Z transition

A 2-aminothieno[3,4-d] pyrimidine G-mimic deoxyribonucleoside, thdG, was synthesized and incorporated readily into oligonucleotides as a versatile fluorescent guanine analogue. We demonstrate that thdG enables the visual detection of Z-DNA successfully based on different pi-stacking of B- and Z-DNA.

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Bibliographic Details
Published inChemical communications (Cambridge, England) Vol. 50; no. 13; pp. 1573 - 1575
Main Authors Park, Soyoung, Otomo, Haruka, Zheng, Linjie, Sugiyama, Hiroshi
Format Journal Article
LanguageEnglish
Published CAMBRIDGE Royal Soc Chemistry 01.01.2014
Subjects
Analogue
Base Sequence
Chemistry
Chemistry, Multidisciplinary
Deoxyguanosine - analogs & derivatives
DNA, B-Form - analysis
DNA, Z-Form - analysis
Emissivity
Fluorescent Dyes - chemistry
Guanines
Oligonucleotides
Oligonucleotides - chemistry
Physical Sciences
Science & Technology
Visual
Visualization
HANDED Z-DNA
NUCLEOTIDES
BASE-PAIR
2-AMINOPURINE FLUORESCENCE
QUADRUPLEX
RNA
NUCLEOSIDES
POLYMERASE
DERIVATIVES
PROBES
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Summary:A 2-aminothieno[3,4-d] pyrimidine G-mimic deoxyribonucleoside, thdG, was synthesized and incorporated readily into oligonucleotides as a versatile fluorescent guanine analogue. We demonstrate that thdG enables the visual detection of Z-DNA successfully based on different pi-stacking of B- and Z-DNA.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:1359-7345
1364-548X
DOI:10.1039/c3cc48297a