Effect of water-soluble polysaccharides from Morchella esculenta on high-fat diet-induced obese mice: changes in gut microbiota and metabolic functions

• Published in: Food & function Vol. 14; no. 11; pp. 5217 - 5231
• Main Authors: Liu, Bingshu, Yu, Leilei, Zhai, Qixiao, Li, Miaoyu, Li, Liuruolan, Tian, Fengwei, Chen, Wei
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 06.06.2023
• Subjects: Animals; CCAAT/enhancer-binding protein; Diet; Diet, High-Fat - adverse effects; Dysbacteriosis; Fatty acids; Gastrointestinal Microbiome; Gene expression; Glucose; Glucose transporter; High fat diet; Injury prevention; Intestinal microflora; Liver; Mannose; Mice; Mice, Inbred C57BL; Mice, Obese; Microbiota; Microorganisms; Morchella esculenta; Obesity; Obesity - etiology; Peroxisome proliferator-activated receptors; Polysaccharides; Polysaccharides - pharmacology; Probiotics; Receptors; Saccharides; Water chemistry
• ISSN: 2042-6496; 2042-650X
• DOI: 10.1039/d3fo00574g

polysaccharides exhibit numerous probiotic activities, but their regulatory effects on the gut microbiota are unclear. This study was conducted to explore whether polysaccharides can regulate dysbacteriosis caused by a high-fat diet and relieve obesity. We extracted a water-soluble polysaccharide from (MPF, purity: 96.19%, consisting of 55.97% glucose, 9.63% xylose, and 22% mannose) that reduces mouse fat accumulation, alleviates obesity, and relieves liver injury, after 90 days of high-fat diet intake. This polysaccharide reversed dysbiosis and regulated the abundance of gut microbiota caused by a high-fat diet (restoring the ratio of Firmicutes/Bacteroidetes and changing the abundances of , , and ), increasing short-chain fatty acids and decreasing gene expression in the liver ( , , ( ) , , , and ). We identified a regulatory relationship between polysaccharides, gut microbiota, and the liver as a potential mechanism by which polysaccharides can alleviate obesity.