Radiation Synthesis of Magnesium Doped Nano Hydroxyapatite/(Acacia-Gelatin) Scaffold for Bone Tissue Regeneration: In Vitro Drug Release Study

• Published in: Journal of inorganic and organometallic polymers and materials Vol. 30; no. 8; pp. 2890 - 2906
• Main Authors: Raafat, Amany I., Kamal, H., Sharada, Hayat M., Abd elhalim, Sawsan A., Mohamed, Randa D.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.08.2020; Springer Nature B.V
• Subjects: Apatite; Biocompatibility; Biodegradability; Biomedical materials; Cementing; Chemical synthesis; Chemistry; Chemistry and Materials Science; Crosslinking; Drug delivery systems; E coli; Functional groups; Gamma irradiation; Gamma rays; Gelatin; Hydroxyapatite; Inorganic Chemistry; Ketoprofen; Magnesium; Mechanical properties; Nanocomposites; Nanoparticles; Organic Chemistry; Polymer Sciences; Porosity; Regeneration; Scaffolds; Sterilization; Tissue engineering; Toxicity; Acacia gum; Radiation copolymerization; Scaffold; Mg-doped hydroxyapatite; Bone regeneration; Gelatin
• ISSN: 1574-1443; 1574-1451
• DOI: 10.1007/s10904-019-01418-3

Novel three-dimensional biodegradable porous nanocomposite bone scaffolds were fabricated using acacia gum and gelatin as the base polymer matrix and magnesium doped nano hydroxyapatite as cementing materials using gamma irradiation facility for crosslinking and sterilization processes. Mg-doped HAp nanoparticles were synthesized using wet chemical method. XRD studies verified the nano-scale size of the prepared HAp. In addition to Ca and P in the prepared n-HAp, the EDX analysis revealed the presence of Mg in the doped HAp samples. FTIR studies confirmed the existence of the characteristic functional groups of the scaffold constituents. The swelling behavior was found to be dependent on the quantity of embedded HAp nanoparticles. Nanocomposite scaffold porosity ranged from 26 to 39%, which increased with the inclusion of Mg ions. The developed scaffolds showed appropriate mechanical properties that enhanced by the existence of HAp nanoparticles. The incorporation of the Mg-doped HAp nanoparticles encourages the development of bone-like apatite layer. In vitro cytotoxicity assessment and blood compatibility demonstrated their biocompatibility. The developed scaffolds show promising antibacterial activity against Staphylococcus aureus and Escherichia coli. In vitro drug release study showed that the loaded Ketoprofen scaffolds were able to deliver the loaded drug sustainably.