A ferrofluid-based homogeneous assay for highly sensitive and selective detection of single-nucleotide polymorphisms

A simple and low-cost colorimetric assay utilizing ferrofluidic nanoparticulate probes (FNPs) and a ligase for single-nucleotide polymorphism genotyping is described. Excellent sensitivity and selectivity were accomplished through the engagement of the FNPs and a ligase chain reaction.

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Bibliographic Details
Published inChemical communications (Cambridge, England) Vol. 49; no. 73; pp. 8114 - 8116
Main Authors Shen, Wei, Lim, Cai Le, Gao, Zhiqiang
Format Journal Article
LanguageEnglish
Published England 21.09.2013
Subjects
Biological Assay - methods
Colorimetry - economics
Colorimetry - methods
Ligases - chemistry
Limit of Detection
Magnetics
Nanoparticles - chemistry
Polymorphism, Single Nucleotide
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Summary:A simple and low-cost colorimetric assay utilizing ferrofluidic nanoparticulate probes (FNPs) and a ligase for single-nucleotide polymorphism genotyping is described. Excellent sensitivity and selectivity were accomplished through the engagement of the FNPs and a ligase chain reaction.
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ISSN:1359-7345
1364-548X
DOI:10.1039/c3cc43281e