Inhalation teratology and two-generation reproduction studies with 1,1-dichloro-1-fluoroethane (HCFC-141 b)

• Published in: Food and chemical toxicology Vol. 33; no. 4; pp. 285 - 300
• Main Authors: Rusch, G.M., Millischer, R.-J., de Rooij, C., Brooker, A.J., Hughes, E., Coombs, D.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.04.1995; New York, NY Elsevier Science
• Subjects: Administration, Inhalation; Animals; Biological and medical sciences; Body Weight - drug effects; Chlorofluorocarbons - administration & dosage; Chlorofluorocarbons - toxicity; Chlorofluorocarbons, Ethane; Eating - drug effects; Embryology: invertebrates and vertebrates. Teratology; Embryonic and Fetal Development - drug effects; ethylene dichloride; Female; fluorine; Fundamental and applied biological sciences. Psychology; Gestational Age; inhalation; Male; Organ Size - drug effects; Pregnancy; Prenatal Exposure Delayed Effects; Rabbits; Rats; Reproduction - drug effects; reproductive disorders; Specific Pathogen-Free Organisms; teratogenesis; Teratogens - toxicity; Teratology. Teratogens; Tissue Distribution; NOEL; CFC; CFC fluid; Rat; Toxicity; Chemical compound; Rodentia; Multigeneration study; Rabbit; Lagomorpha; Halocarbon; Inhalation; Vertebrata; Mammalia; Animal; Teratogen; Substitute
• ISSN: 0278-6915; 1873-6351
• DOI: 10.1016/0278-6915(94)00144-D

HCFC-141b is one of the chemicals being considered as a replacement for CFC 11 in solvent and foam-blowing applications. Teratology studies were conducted in both rats and rabbits and a two-generation reproduction inhalation toxicity study was conducted in rats. The pregnant rabbits were exposed to levels of 0 (control), 1400, 4200 and 12,600 ppm HCFC-141b from day 7 today 19 of gestation (6 hr/day). There was no evidence of developmental or teratogenic effects on the foetuses. The pregnant rats in the teratology study were exposed to levels of 0 (control), 3200, 8000 and 20,000 ppm from days 6 to 15 of gestation (6 hr/day). In the 20,000 ppm exposure group, there was an increase in implantation losses; furthermore, in this group, foetal weights tended to be lower than controls. As with the rabbits, there was no evidence of a teratogenic effect. The reproduction study was conducted at exposure levels of 0, 2000, 8000 and 20,000 ppm, 7 days/wk starting approximately 10 wk before the first pairing. Adult rats exposed at 20,000 ppm (and, to a lesser extent, those exposed to 8000 ppm) showed increases in water intake, slight increases in food consumption, and decreases in body weight. Following the mating of the F0 parents, there were fewer litters in the 20,000 ppm exposure level group than in controls. When these parents were then paired with different partners, again, the number of litters was lower in the 20,000 ppm group, although most of the animals that did not produce litters the first time mated successfully the second time. When the F1 animals were mated to produce the second generation, the number of litters was comparable for all groups. In the second F0 mating and the F1 mating, the number of pups per litter was lower at 20,000 ppm; although birth weights were comparable, body weight gain tended to be slower in the high-level exposure group. Survival was good in all groups. At 8000 ppm no significant effects were observed in the pups and only minimal signs in the adults. The 2000 ppm exposure level represented a clear no-observed-effect level for all indices.