The repertoire and levels of secondary metabolites in microbial cocultures depend on the inoculation ratio: a case study involving Aspergillus terreus and Streptomyces rimosus
Objective The aim of this study was to determine the influence of the inoculation volume ratio on the production of secondary metabolites in submerged cocultures of Aspergillus terreus and Streptomyces rimosus . Results The shake flask cocultures were initiated by using 23 inoculum variants that inc...
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Published in | Biotechnology letters Vol. 46; no. 4; pp. 601 - 614 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Dordrecht
Springer Netherlands
01.08.2024
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Objective
The aim of this study was to determine the influence of the inoculation volume ratio on the production of secondary metabolites in submerged cocultures of
Aspergillus terreus
and
Streptomyces rimosus
.
Results
The shake flask cocultures were initiated by using 23 inoculum variants that included different volumes of
A. terreus
and
S. rimosus
precultures. In addition, the axenic controls were propagated in parallel with the cocultures. UPLC‒MS analysis revealed the presence of 15 secondary metabolites, 12 of which were found both in the “
A. terreus
vs.
S. rimosus
” cocultures and axenic cultures of either
A. terreus
or
S. rimosus
. The production of the remaining 3 molecules was recorded solely in the cocultures. The repertoire and quantity of secondary metabolites were evidently dependent on the inoculation ratio. It was also noted that detecting filamentous structures resembling typical morphological forms of a given species was insufficient to predict the presence of a given metabolite.
Conclusions
The modification of the inoculation ratio is an effective strategy for awakening and enhancing the production of secondary metabolites that are not biosynthesized under axenic conditions. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0141-5492 1573-6776 1573-6776 |
DOI: | 10.1007/s10529-024-03500-4 |