Auxin herbicides induce H2O2 overproduction and tissue damage in cleavers (Galium aparine L.)

• Published in: Journal of experimental botany Vol. 52; no. 362; pp. 1811 - 1816
• Main Authors: Grossmann, Klaus, Kwiatkowski, Jacek, Tresch, Stefan
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.09.2001
• Subjects: (+)‐abscisic acid; 1‐aminocyclopropane‐1‐carboxylic acid; ABA; ACC; auxin herbicides; Biological and medical sciences; carbon assimilation; Chemical control; deoxyribonuclease; DNase; ethylene; Fundamental and applied biological sciences. Psychology; Galium aparine; hydrogen peroxide; Parasitic plants. Weeds; Phytopathology. Animal pests. Plant and forest protection; reactive oxygen species; ROS; senescence; Weeds; Senescence; Auxin; Ethylene; Biosynthesis; Pyridine derivatives; Dose activity relation; Regulation(control); Dicotyledones; Sesquiterpenes; Angiospermae; Rubiaceae; Stomatal movement; Abscisic acid; Plant growth substance; Mechanism of action; Chlorophyll; Weed; Picolinic acid derivatives; Pesticides; Quinoline derivatives; Plant leaf; Hydrogen Peroxides; Herbicide; Phytotoxicity; Picloram; Photosynthetic pigment; Spermatophyta; Photosynthesis; Chemical weed control
• ISSN: 0022-0957; 1460-2431
• DOI: 10.1093/jexbot/52.362.1811

The phytotoxic effects of auxin herbicides, including the quinoline carboxylic acids quinmerac and quinclorac, the benzoic acid dicamba and the pyridine carboxylic acid picloram, were studied in relation to changes in phytohormonal ethylene and abscisic acid (ABA) levels and the production of H2O2 in cleavers (Galium aparine). When plants were root‐treated with 10 μM quinmerac, ethylene synthesis was stimulated in the shoot tissue, accompanied by increases in immunoreactive levels of ABA and its precursor xanthoxal. It has been demonstrated that auxin herbicide‐stimulated ethylene triggers ABA biosynthesis. The time‐course and dose‐response of ABA accumulation closely correlated with reductions in stomatal aperture and CO2 assimilation and increased levels of hydrogen peroxide (H2O2), deoxyribonuclease (DNase) activity and chlorophyll loss. The latter parameters were used as sensitive indicators for the progression of tissue damage. On a shoot dry weight basis, DNase activity and H2O2 levels increased up to 3‐fold, relative to the control. Corresponding effects were obtained using auxin herbicides from the other chemical classes or when ABA was applied exogenously. It is hypothesized, that auxin herbicides stimulate H2O2 generation which contributes to the induction of cell death in Galium leaves. This overproduction of H2O2 could be triggered by the decline of photosynthetic activity, due to ABA‐mediated stomatal closure.