Quantitative analysis of bile acids and their conjugates in duodenal aspirate by fluorimetry after cellulose acetate electrophoresis

Duodenal aspirates are directly applied to cellulose acetate without any previous purification. Bile salts are separated by electrophoresis into seven bands. After enzymatic reaction, quantitative fluorimetric determination was performed on each band of non-sprayed cellulose acetate strips.

Saved in:
Bibliographic Details
Published inClinica chimica acta Vol. 71; no. 1; pp. 27 - 33
Main Authors Beke, R., De Weerdt, G.A., Parijs, J., Barbier, F.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 16.08.1976
Subjects
Bile Acids and Salts - analysis
Bile Acids and Salts - metabolism
Chromatography, Gas
Chromatography, Thin Layer
Duodenum - metabolism
Electrophoresis, Cellulose Acetate - methods
Humans
Methods
Spectrometry, Fluorescence
Taurocholic Acid - analysis
Online AccessGet full text

Cover

More Information
Summary:Duodenal aspirates are directly applied to cellulose acetate without any previous purification. Bile salts are separated by electrophoresis into seven bands. After enzymatic reaction, quantitative fluorimetric determination was performed on each band of non-sprayed cellulose acetate strips.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0009-8981
1873-3492
DOI:10.1016/0009-8981(76)90271-0