Rate and extent of enzymatic lipolysis at subfreezing temperatures

Little attention has been given to the effects that various freezing treatments have on rates of enzyme-catalyzed reactions in frozen systems and to the relationship between subfreezing temperatures and the ultimate extent to which a given reaction proceeds. Both of these aspects were explored using...

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Bibliographic Details
Published inCryobiology Vol. 15; no. 2; pp. 199 - 204
Main Authors Parducci, L.G., Fennema, O.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier Inc 01.04.1978
Subjects
Buffers
Freezing
Kinetics
Lipase - metabolism
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Summary:Little attention has been given to the effects that various freezing treatments have on rates of enzyme-catalyzed reactions in frozen systems and to the relationship between subfreezing temperatures and the ultimate extent to which a given reaction proceeds. Both of these aspects were explored using a model system consisting of lipase in an emulsion of tributyrin in water. The ultimate extent to which tributyrin was hydrolyzed decreased from 5.4% at −2 °C to 4.0% at −12 °C. Hydrolysis proceeded almost to completion at temperatures above 0 °C. Rapid freezing to −80 °C produced a substantially slower initial reaction rate at −8 °C than rapid freezing to −20 °C, or than slow freezing, regardless of the temperature nadir.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0011-2240
1090-2392
DOI:10.1016/0011-2240(78)90024-X