Electrochemical immunosensor based on AuNPs/ERGO@CNT nanocomposites by one-step electrochemical co-reduction for sensitive detection of P-glycoprotein in serum

• Published in: Biosensors & bioelectronics Vol. 222; p. 115001
• Main Authors: Lu, Tai-Cheng, Sun, Yi-Ming, Zhong, Yu, Lin, Xin-Hua, Lei, Yun, Liu, Ai-Lin
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 15.02.2023
• Subjects: ATP Binding Cassette Transporter, Subfamily B; ATP Binding Cassette Transporter, Subfamily B, Member 1; Biosensing Techniques - methods; Carbon nanotubes; Electrochemical immunosensor; Electrochemical Techniques - methods; Gold; Gold nanoparticles; Graphene oxide; Graphite; Immunoassay - methods; Limit of Detection; Metal Nanoparticles; Nanocomposites; P-glycoprotein; P-glycoprotein; Carbon nanotubes; Electrochemical immunosensor; Graphene oxide; Gold nanoparticles
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2022.115001

P-glycoprotein (P-gp), a transmembrane glycoprotein widely expressed on the surface of various cells, is highly associated with multidrug resistance (MDR) that heralds the malignant progress of disease after drug treatment. Notably, there have been reported that serum P-gp is a potential marker for assessing the progression of disease resistance. Currently, there are few methods for point-of-care serum P-gp detection. In this study, we proposed a gold nanoparticles/electrochemically reduced graphene oxide@carbon nanotube (AuNPs/ERGO@CNT) modified immunosensor based on a one-step electrochemical co-reduction method. The limit of detection (LOD) of our constructed electrochemical immunosensor for P-gp detection reached 0.13 ng/mL, and the detection results in serum were consistent with ELISA. The developed immunosensor is expected to provide a scientific basis for the clinical application of serum P-gp monitoring and integrated medicine.