Identification of two clock proteins in acetabularia cliftonii and construction of cDNA libraries from Acetabularia cliftonii and Acetabularia mediterranea

• Published in: International journal of biochemistry Vol. 24; no. 7; pp. 1141 - 1150
• Main Authors: Yang, Xiao-Ping, De Groot, Egon J.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier B.V 1992; Pergamon
• Subjects: Acetabularia - chemistry; Acetabularia - genetics; Acetabularia - metabolism; algae and seaweeds; Analytical, structural and metabolic biochemistry; Base Sequence; Biological and medical sciences; circadian rhythm; Circadian Rhythm - genetics; cloning; DNA - genetics; DNA libraries; Fundamental and applied biological sciences. Psychology; Gene Library; Miscellaneous; Molecular Sequence Data; Nucleic Acid Hybridization; nucleotide sequences; periodicity; plant proteins; Plant Proteins - analysis; Plant Proteins - biosynthesis; Plant Proteins - genetics; Poly A - genetics; Poly A - isolation & purification; Protein Biosynthesis - genetics; protein synthesis; Proteins; RNA - isolation & purification; Nucleotide sequence; Algae; Chlorophycophyta; Homology; Acetabularia mediterranea; Biosynthesis; Biological rhythm; Gene library; Chlamydomonas; Proteins; Characterization; Molecular cloning; Thallophyta
• ISSN: 0020-711X
• DOI: 10.1016/0020-711X(92)90385-E

1. 1. Two clock proteins were identified in A. cliftonii. The first has a molecular weight (mol. wt) of 200 kDa (P200) and its synthesis shows a 24 hr periodicity. The second has mol. wt of 130 kDa (P 130) and shows a semicircadian rhythm with a periodicity of about 12hr, 2. 2. cDNA libraries from A. cliftonii and A. mediterranea were prepared by cloning cDNA in λ gt10 and Agtil, respectively. 3. 3. One clone each of the two libraries hybridized with the human β-actin pseudogene. One clone of the A. mediterranea and 4 clones of the A. cliftonii libraries hybridized to Chlamydomonas heat-shock gene.