Fast Screening of Tissue Samples for Glycogen

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 135; pp. 16 - 19
• Main Authors: Stefan-van Staden, Raluca-Ioana, Diaconeasa, Amalia Gabriela, Stanciu-Gavan, Camelia
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 20.02.2017
• Subjects: Biosensing Techniques - instrumentation; Biosensing Techniques - methods; Colon - chemistry; Diamond - chemistry; Electrodes; Glycogen; Glycogen - analysis; Humans; Needle stochastic sensor; Oils - chemistry; Paraffin - chemistry; Polysaccharides - chemistry; Screening system; Skin - chemistry; Time Factors; Tissue sample; Screening system; Needle stochastic sensor; Tissue sample; Glycogen
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/j.jpba.2016.12.009

•Two stochastic needle sensors were designed for the assay of glycogen in tissues.•The sensors exhibited high sensitivities and low limits of determination for glycogen.•The lower limit of determination recorded was 1fmol/L. Screening of tissue samples for glycogen is very important in assessing the ageing, but also the state of health of the tissue. Therefore, two needle stochastic sensors based on maltodextrins presenting different dextrose equivalence (DE) MD-m (DE 13.0-17.0), and MD-M (DE 16.5-19.5) immobilized in diamond paste (obtained from synthetic diamond and paraffin oil) were designed and characterized. These stochastic sensors were used reliable for both qualitative and quantitative analysis for the assay of glycogen in tissue samples with limits of determination as low as 1fmol L−1.