Association of Interleukin-10 –592 C > A gene polymorphism with coronary artery disease: A case-control study and meta-analysis

• Published in: Cytokine (Philadelphia, Pa.) Vol. 139; p. 155403
• Main Authors: Ghalandari, Marzieh, Jamialahmadi, Khadijeh, Nik, Maryam Mardan, Pirhoushiaran, Maryam, Mirhafez, Seyed Reza, Rooki, Hassan, Avan, Amir, Ghazizadeh, Hamideh, Moohebati, Mohsen, Nohtani, Mahdi, Zaimkohan, Hooshang, Ferns, Gordon A., Pasdar, Alireza, Ghayour-Mobarhan, Majid
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.03.2021
• Subjects: Alleles; Atherosclerosis - genetics; Case-Control Studies; Coronary artery disease; Coronary Artery Disease - genetics; Cross-Sectional Studies; Female; Genetic Predisposition to Disease - genetics; Genotype; Humans; Inflammation, Meta-analysis; Interleukin-10 - genetics; Interleukin-10 –592 C > A polymorphism; Iran; Male; Middle Aged; Polymorphism, Restriction Fragment Length - genetics; Polymorphism, Single Nucleotide - genetics; Iran; Interleukin-10 –592 C > A polymorphism; Coronary artery disease; Inflammation, Meta-analysis
• ISSN: 1043-4666; 1096-0023
• DOI: 10.1016/j.cyto.2020.155403

•The cross-sectional study supports a significant association of IL-10 –592 C > A with CAD.•The subjects carrying A allele had a significantly higher risk of CAD.•The subjects carrying AA genotype had a significantly higher risk of CAD.•The results of the meta-analysis showed a significant association between IL and 10 –592 C > A and CAD in the co-dominant model. Coronary-artery-disease (CAD) is the leading cause of death worldwide, and hence there is a need to identify reliable markers for identifying individuals at high risk of developing CAD. Interleukin-10 (IL-10) is an anti-inflammatory cytokine that is associated with an increased risk of developing both atherosclerosis and acute coronary events. The study aimed to explore the association of a genetic variant in IL-10 with the risk of developing CAD and the severity of the disease. To further explore, a systematic review and meta-analysis was performed. The cumulative results of the relationship between IL and 10 –592 C > A polymorphism and CAD in Iranian population have also been presented. In this cross sectional study, a total of 948 individuals including 307 healthy controls and 641 patients that among cases, four hundred and fifty-five of the patients had > 50% stenosis (angiogram positive group) and 186 patients had < 50% stenosis (angiogram negative group) were recruited from the Mashhad-Stroke and Heart-Atherosclerotic-Disorders cohort. Genotyping for the IL-10 –592 C > A polymorphism was performed using a PCR-RFLP technique, and statistical analysis undertaken by univariate and multivariate analyses. PubMed, Google Scholar and Scopus were searched for papers related to this polymorphism up to October 2019. The Meta-analysiswas done based on the random effect model using a Meta-analysis. In our study, the frequency of the variant A allele of the IL-10 –592 C > A was significantly higher in CAD patients than the control group (P value = 0.043). Moreover, subjects carrying AA genotype had a significantly higher risk of CAD (OR: 1.8, 95%CI: 1.04–3.16), p = 0.03), compared to those with the wild type genotype. The results of meta-analysis of 9336 cases and 8461 controls did not also show any significant association between IL and 10 –592 C > A and CAD in dominant and recessive genetic models but only in co-dominant model when fix effect was applied. Although our research findings support a significant association of genetic polymorphism in the IL10 gene with cardiovascular diseases, this finding cannot be confirmed in meta-analysis. Further functional analysis and evaluation of this marker in a multicenter setting are needed to establish its value as a risk stratification marker.