miR-6089 may prevent the inflammatory events leading to cardiovascular disorders in RA patients

• Published in: Heliyon Vol. 10; no. 17; p. e36763
• Main Authors: Shamsi, Afsaneh, Roghani, Seyed Askar, Shamsi, Mohammad, Jalili, Cyrus, Taghadosi, Mahdi, Soufivand, Parviz
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 15.09.2024; Elsevier
• Subjects: Cardiovascular disease; Cardiovascular risk factors; LncRNA-HIX003209; miR-6089; Rheumatoid arthritis; VSMCs; CXCL9; SCORE; BPS; FRS; LncRNA; Cardiovascular disease; RA; CVD; NT-proBNP; Cardiovascular risk factors; Rheumatoid arthritis; CXCR3; BPD; miRNA; HS-CRP; LncRNA-HIX003209; miR-6089
• ISSN: 2405-8440; 2405-8440
• DOI: 10.1016/j.heliyon.2024.e36763

Cardiovascular disease (CVD) is the most important comorbid condition in rheumatoid arthritis (RA) patients. Dysregulated expression of non-coding RNA families has a critical role in RA-associated inflammatory events, including cardiovascular manifestations. The long non-coding RNA (lncRNA)- HIX003209 has a role in RA associated inflammation. In the current study, we investigated the association of HIX003209 and its downstream microRNA, miR-6089, with various cardiovascular and inflammatory biomarkers in RA patients. 60 RA patients, including 30 newly diagnosed and 30 on-treatment patients were recruited in this study, and 30 healthy people were selected as a control group. The gene expression of HIX003209, miR-6089, and CXCR3 were measured using Real-time PCR. The CVD risk was measured using Systematic Coronary Risk Evaluation (SCORE) and Framingham Risk Score (FRS). The gene expression of LncRNA-HIX003209 was elevated significantly in newly-diagnosed compared to under-treatment and control groups (p < 0.05). The miR-6089 gene expression was elevated significantly in under-treatment RA patients group compared to control group (p < 0.001). There was a significant positive correlation between LncRNA-HIX003209 with CXCR3 gene expression (p < 0.01, r = 0.341). There was a significantly negative correlation between the gene expression of miR-6089 with DAS-28 (p < 0.05, r = −0.309), NT-proBNP plasma level (p = 0.039, r = −0.268), and CXCL9 plasma level (p < 0.001, r = −0.421). Regarding its anti-inflammatory effects, miR-6089 may play an important role in preventing the pathological events of cardiovascular disorders in RA patients, through its inhibitory effects on inflammatory chemokines, such as CXCL9, and NT-ProBNP. Higher expression of LncRNA-HIX003209 may disrupt the anti-inflammatory effect of miR-6089 in RA patients.