A comparative study of the expression patterns of Fign family members in zebrafish embryonic development

• Published in: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology Vol. 251; p. 110522
• Main Authors: Dong, Zhangji, Li, Yuanyuan, Chen, Xu, Lai, Xiaona, Liu, Mei
• Format: Journal Article
• Language: English
• Published: England Elsevier Inc 01.01.2021
• Subjects: Amino Acid Sequence; Animals; Computational Biology; Embryonic Development - genetics; Fidgetin like 1 (fignl1); Fidgetin like 2 (fignl2); Fign; Gene Expression Regulation, Developmental; In situ hybridization (ISH); Microtubule-Associated Proteins - chemistry; Microtubule-Associated Proteins - genetics; Microtubule-Associated Proteins - metabolism; Zebrafish - embryology; Zebrafish - genetics; Zebrafish embryonic development; Zebrafish embryonic development; Fidgetin like 1 (fignl1); Fign; Fidgetin like 2 (fignl2); In situ hybridization (ISH)
• ISSN: 1096-4959; 1879-1107
• DOI: 10.1016/j.cbpb.2020.110522

During development, highly dynamic reconstruction of microtubules is involved in many cellular processes, including cell division, migration, morphological changes, and material transportation within cells. Microtubule severing proteins (MSPs), with the function of cutting microtubules into short parts, are important regulators in the reconstruction of microtubule arrays. Fidgetin (fign) and its family members fidgetin like 1 (fignl1) and fignl2 are MSPs, and knowledge on the expression patterns of fign family members will benefit our understanding of their primary roles in one specific stage during development. In this study, we compared the evolutionary relationships of fign family members and found that fignl2 is closer to fign than fignl1. We utilized the zebrafish model and in situ hybridization (ISH) to parallelly identify the expression features of fign family members. Our findings revealed that before 12 h post fertilization (hpf), the expression patterns of fign and fignl1 and fignl2 genes were similar, but differences arose thereafter. Fignl2 transcripts were present in more tissues and organs of zebrafish after 12 hpf and potentially exhibited more ubiquitous functions. This study is the first to assess systematic comparable data on the expression patterns of fign family members during development [Display omitted] •A systematic study of expression of fign family members in zebrafish development.•Expression patterns of fign family members suggest functional differentiation.•Expression of fignl2 exists in tissues other than the nervous system.