CHD5 is a potential tumor suppressor in non small cell lung cancer (NSCLC)

• Published in: Gene Vol. 618; pp. 65 - 68
• Main Authors: Baykara, Onur, Tansarikaya, Merve, Bulut, Pelin, Demirkaya, Ahmet, Buyru, Nur
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 30.06.2017
• Subjects: Carcinoma, Non-Small-Cell Lung - genetics; Carcinoma, Non-Small-Cell Lung - metabolism; Carcinoma, Non-Small-Cell Lung - pathology; CHD5; Chromodomain; DNA Helicases - genetics; DNA Helicases - metabolism; DNA Methylation; Female; Humans; Lung cancer; Lung Neoplasms - genetics; Lung Neoplasms - metabolism; Lung Neoplasms - pathology; Male; Middle Aged; Nerve Tissue Proteins - genetics; Nerve Tissue Proteins - metabolism; NSCLC; Promoter Regions, Genetic; CHD5; MS-PCR; NSCLC; RT-PCR; Lung cancer; Chromodomain
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/j.gene.2017.04.010

Lung cancer is one of the deadliest types of cancers and genetic and epigenetic alterations play major roles in its development. Chromodomain (CHD) protein family acts in chromatin organization, regulation of transcription and also genomic stability and cancer prevention. Although CHD5, a member of this family was shown to contribute to major cellular events and functions as a tumor suppressor gene in various types of cancer, it is not clear whether CHD5 plays a role in lung carcinogenesis. The aim of this study was to investigate the possible role of CHD5 in progression of non-small cell lung cancer (NSCLC). Expression levels of CHD5 gene in 59 tumor and corresponding non-cancerous lung tissue samples were analyzed by qRT-PCR and the methylation status of the promoter region was investigated by methylation specific PCR (MS-PCR). The Akt phosphorylation levels were investigated by Western Blot (WB). CHD5 was down-regulated in 17 (39.5%) and up-regulated in 24 (55.8%) of tumor specimens. Even though the promoter of CHD5 was hypermethylated in 8 patients, it was not found associated with CHD5 gene expression (p=0.08). Akt phosphorylation was increased in 14 (53.8%) and decreased in 12 (46.2%) of the samples but no significant association was found between p-Akt phosphorylation and CHD5 expression (p=0.67). We suggest that CHD5 may act as a tumor suppressor gene in NSCLC. •The methylation and expression levels of CHD5 gene is investigated in non-small cell lung cancer patients.•CHD5 was down-regulated in 39.5% and up-regulated in 55.8% of the patients.•CHD5 promoter was methylated in 26% of the patients.•No association was found between methylation and expression patterns.•We propose CHD5 acts as a tumor suppressor gene in NSCLC.