Dual activity of Meloidogyne incognita-regulated Musa acuminata Pathogenesis-related-10 (MaPR-10) gene

• Published in: Gene Vol. 809; p. 146041
• Main Authors: Rajendram, Arullthevan, Mostaffa, Nur Hikmah, Dumin, Walftor, Oke, Mushafau Adebayo, Simarani, Khanom, Somasundram, Chandran, Razali, Zuliana, Rejab, Nur Ardiyana, Al-Idrus, Aisyafaznim
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 30.01.2022
• Subjects: Animals; Antifungal Agents - pharmacology; Antifungal enzyme; Aspergillus fumigatus; Aspergillus fumigatus - drug effects; Aspergillus niger - drug effects; Gene Expression Regulation, Plant; Host-Parasite Interactions - genetics; Meloidogyne incognita; Musa - genetics; Musa - parasitology; Musa spp; Onions - genetics; Pathogenesis-related-10 gene; Phylogeny; Plant Immunity - genetics; Plant Proteins - genetics; Plant Proteins - metabolism; Plant Proteins - pharmacology; Plant Roots - genetics; Plant Roots - parasitology; Plants, Genetically Modified; Ribonuclease; Tylenchoidea - pathogenicity; β-1,3-Glucanase; LiCl; PR; PR10; PTI; RNA; SAR; DNS; ITS; HR; mRNA; Ribonuclease; CTAB; SA; β-1,3-Glucanase; PR1-like; Meloidogyne incognita; cDNA; dpi; RNase; MgCl2; SDS; NaOAc; EtdBr; GH16; dNTPs; PR1; PAMP; Musa spp; MgSO4; RE; MaPR10; RKN; Antifungal enzyme; Pathogenesis-related-10 gene; DNA; JA; sdH2O; IPTG; NaCl; ETI; Aspergillus fumigatus; PCR
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/j.gene.2021.146041

•Pathogenesis-related- 10 (PR10) gene from Musa acuminata cultivars was characterised.•Novel finding on M. acuminata PR10 protein as β-1,3-glucanase and ribonuclease.•Novel finding on antagonistic effect of plant-based proteins on Aspergillus fumigatus. Plant immunity to pathogen infections is a dynamic response that involves multiple organelles and defence signalling systems such as hypersensitive response (HR) and systemic acquired resistance (SAR). The latter requires the function of Pathogenesis-related (PR) proteins, a common plant protein family with diverse roles in plant innate immunity. Our previous proteomics study showed that a PR gene (ITC1587_Bchr9_P26466_MUSBA) was differentially regulated during a compatible banana-M. incognita interaction, substantiating the isolation of this gene in the current study. Here, we successfully isolated and characterised Pathogenesis-related-10 (PR10) gene with β-1,3-glucanase and ribonuclease (RNase) activities from two Musa acuminata cultivars (denoted as MaPR10) namely Berangan and Grand Naine (ITC1256). We found that MaPR10 cloned sequences possess glycine-rich loop domain and shared conserved motifs specific to PR10 gene group, confirming its identity as a member of this group. Interestingly, we also found a catalytic domain sequence for glycoside hydrolase family 16 (EXDXXE), unique only to MaPR10 cloned sequences. Two peptide variants closely related to the reference sequence ITC1587_Bchr9_P26466_MUSBA namely MaPR10-BeB5 and MaPR10-GNA5 were overexpressed and purified to test for their functionality. Here, we confirmed that both protein variants possess β-1,3-glucanase and ribonuclease (RNase) activities, and inhibit the growth of Aspergillus fumigatus, a human opportunistic pathogen. To our knowledge, this is the first PR10 plant proteins with such properties to be reported thus far.