Real-time imaging of alkaline phosphatase activity of diabetes in mice via a near-infrared fluorescent probe

• Published in: Chemical communications (Cambridge, England) Vol. 57; no. 4; pp. 480 - 483
• Main Authors: Wang, Wen-Xin, Jiang, Wen-Li, Guo, Hong, Li, Yongfei, Li, Chun-Yan
• Format: Journal Article
• Language: English
• Published: CAMBRIDGE Royal Soc Chemistry 14.01.2021; Royal Society of Chemistry
• Subjects: Alkaline phosphatase; Alkaline Phosphatase - metabolism; Animals; Cell Line, Tumor; Chemistry; Chemistry, Multidisciplinary; Diabetes; Diabetes Mellitus, Experimental - enzymology; Fluorescent Dyes - chemical synthesis; Fluorescent Dyes - chemistry; Fluorescent Dyes - toxicity; Fluorescent indicators; Fluorometry; Humans; Limit of Detection; Mice; Physical Sciences; Quinolinium Compounds - chemical synthesis; Quinolinium Compounds - chemistry; Quinolinium Compounds - toxicity; Real time; Science & Technology; Selectivity; Spectrometry, Fluorescence; Synthesis; Xanthenes - chemical synthesis; Xanthenes - chemistry; Xanthenes - toxicity; ISOENZYME; ISOZYMES; ASSAY
• ISSN: 1359-7345; 1364-548X
• DOI: 10.1039/d0cc07292c

A novel water-soluble near-infrared fluorescent probe named QX-P with simple synthesis is developed. QX-P has high sensitivity and selectivity to ALP. Moreover, the probe can not only visualize ALP activity in four cell lines, but also real-time image ALP activity during the diagnosis and treatment of diabetes in mice.