Evaluation of adenosine deaminase in saliva and serum, and salivary α-amylase, in canine pyometra at diagnosis and after ovariohysterectomy

• Published in: The veterinary journal (1997) Vol. 236; pp. 102 - 110
• Main Authors: Tecles, F., Escribano, D., Contreras-Aguilar, M.D., Rubio, C.P., Szczubiał, M., Cerón, J.J., Dąbrowski, R., Tvarijonaviciute, A.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.06.2018
• Subjects: Adenosine deaminase; Adenosine Deaminase - analysis; Animals; Bitch; Dog Diseases - diagnosis; Dog Diseases - enzymology; Dogs; Female; Hysterectomy - veterinary; Ovariectomy - veterinary; Pyometra; Pyometra - diagnosis; Pyometra - enzymology; Pyometra - veterinary; Saliva; Saliva - enzymology; Salivary alpha-Amylases - analysis; α-Amylase; Bitch; α-Amylase; Adenosine deaminase; Pyometra; Saliva
• ISSN: 1090-0233; 1532-2971
• DOI: 10.1016/j.tvjl.2018.04.018

•Adenosine deaminase (ADA) and α-amylase (α-A) activities were measured in saliva of bitches with pyometra.•Validation of the ADA assay provided<12% imprecision, r2>0.90 in linearity under dilution and 99.2–114.4% recovery.•ADA activity is higher in saliva of bitches with pyometra than in healthy bitches and correlates with inflammatory markers.•Salivary α-A was not increased in bitches with pyometra. An assay for adenosine deaminase (ADA) was validated in serum and saliva in dogs. Changes in ADA and salivary α-amylase activities were analysed in 26 bitches diagnosed with pyometra and compared with activities in 19 healthy bitches. All animals were classified according to the American Society of Anaesthesiologists (ASA) scoring for physical status. In the validation study, the ADA assay had an imprecision<12% and determination coefficients>0.90 in linearity under dilution experiments, with recoveries of 99.2–114.4%. On the day of presentation, salivary ADA activity was significantly higher in dogs with pyometra than in healthy dogs (median values 7.1IU/L vs. 0.8IU/L, respectively; P<0.01). ADA had a moderate positive correlation with leucocyte and band neutrophil counts, haptoglobin, salivary α-amylase and ASA score, and a low positive correlation with C-reactive protein. There were no significant differences in salivary α-amylase activity between dogs with pyometra and healthy dogs (57.3IU/L vs. 27.4IU/L, respectively). Salivary α-amylase had a low correlation with ASA grade, and leucocyte and band neutrophil counts. In 7/26 bitches with pyometra that were sampled 3 and 10days after ovariohysterectomy, there were no significant changes in α-amylase or ADA activities. These results indicate that ADA activity is increased in the saliva of bitches with pyometra, probably related to systemic inflammation.