CircSAMD4A accelerates cell proliferation of osteosarcoma by sponging miR-1244 and regulating MDM2 mRNA expression

• Published in: Biochemical and biophysical research communications Vol. 516; no. 1; pp. 102 - 111
• Main Authors: Yanbin, Zhao, Jing, Zhang
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 13.08.2019
• Subjects: circSAMD4A; MDM2; miR-1244; Osteosarcoma; Proliferation; circSAMD4A; MDM2; Proliferation; miR-1244; Osteosarcoma
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2019.05.182

Here, we firstly examined the proliferation and invasion capacities of different osteosarcoma (OS) cell lines, and analyzed the profiling of the differentially expressed circular RNAs(circRNAs). Then, we identified a novel circRNA(circ_101356/circ_0004846, we named circSAMD4 in the present study) and found it was highly expressed in the OS tissues compared to adjacent non-cancerous tissues. Furthermore, we indicated that circSAMD4A promoted OS proliferation in vivo and in vitro. In addition, we proved that circSAMD4A enhances osteosarcoma cells stemness features. In mechanism, circSAMD4A could sponge miR-1244 in OS cells. Moreover, we verified that MDM2 was a target of miR-1244. In conclusion, circSAMD4A/miR-1244/MDM2 regulatory loop might be a promising therapeutic target for OS treatment. •We identified a novel circular RNA, circSAMD4A in osteosarcoma cells.•circSAMD4 was significantly upregulated in osteosarcoma tissues.•circSAMD4 sponges miR-1244 in osteosarcoma.•MiR-1244 directly targets MDM2 in osteosarcoma.•circSAMD4/miR-1244/MDM2 axis plays critical roles in osteosarcoma pathogenesis.