LncRNA PCAT6 promotes tumor progression in osteosarcoma via activation of TGF-β pathway by sponging miR-185-5p

• Published in: Biochemical and biophysical research communications Vol. 521; no. 2; pp. 463 - 470
• Main Authors: Zhu, Chengdong, Huang, Linhai, Xu, Fei, Li, Ping, Li, Pengfei, Hu, Fangyong
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 08.01.2020
• Subjects: miR-185-5p; Osteosarcoma; PCAT6; TGF-β signaling; TGFBR1; TGFBR2; ceRNA; lncRNA; OS; Osteosarcoma; PCAT6; TGF-β signaling; qRT-PCR; RIP; miR-185-5p; TGFBR1; TGFBR2
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2019.10.136

Long noncoding RNAs (lncRNAs) play crucial roles in tumor development of osteosarcoma (OS). LncRNA PCAT6 was involved in the progression of multiple human cancers. However, the biological function of PCAT6 in OS remains largely unknown. We found that PCAT6 was elevated in OS tissues relative to that in their adjacent normal tissues. The upregulation of PCAT6 was positively associated with metastasis status and advanced stages and predicted poor overall and progression-free survivals in patients with OS. Functionally, silencing PCAT6 inhibited the proliferation, migration and invasion abilities of OS cells. Mechanistically, PCAT6, acting as a competitive endogenous RNA, upregulated expression of TGFBR1 and TGFBR2 to activate TGF-β pathway via sponging miR-185–5p. This study uncovers a novel underlying molecular mechanism of PCAT6-miR-185-5p-TGFBR1/2-TGF-β signaling axis in promoting tumor progression in OS, which indicates that PCAT6 may serve as a promising prognostic factor and therapeutic target again OS. •PCAT6 was upregulated in osteosarcoma (OS) tissues.•The upregulation of PCAT6 was associated with metastasis status, advanced stages and poor prognosis in patients with OS.•The upregulation of PCAT6 promoted OS cell proliferation, migration and invasion.•PCAT6 upregulated expression of TGFBR1 and TGFBR2 to activate TGF-β pathway via sponging miR-185-5p.