The effect of aging on sister chromatid exchange
The advent of the bromodeoxyuridine(BrdU)-differential staining techniques has greatly facilitated the detection of sister chromatid exchanges (SCE). These SCE have been demonstrated to be an accurate reflection of DNA damage both in vitro in cultured cells and in vivo in mouse and rat bone marrow a...
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Published in | Mechanisms of ageing and development Vol. 9; no. 3; pp. 303 - 311 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Ireland
Elsevier Ireland Ltd
01.02.1979
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Subjects | |
Online Access | Get full text |
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Summary: | The advent of the bromodeoxyuridine(BrdU)-differential staining techniques has greatly facilitated the detection of sister chromatid exchanges (SCE). These SCE have been demonstrated to be an accurate reflection of DNA damage both
in vitro in cultured cells and
in vivo in mouse and rat bone marrow and spleen cells. In this review, we examine the effect of cellular aging on both baseline and mutagen-induced SCE levels. In all systems examined, aging did not appear to significantly affect the baseline levels of SCE. However, in human fibroblast cultures we have found a significant decrease in the levels of mutagen-induced SCE as a function of both
in vitro passage level (
in vitro aging) and the age of the cell culture donor (
in vivo aging). In addition we have found a similar decrease in mutagen-induced SCE levels in both mouse and rat bone marrow cells and mouse spleen cells where examinations were performed entirely
in vivo. Diminished mutagen-induced SCE levels were obtained with a wide variety of agents including mitomycin-C, cyclophosphamide, adriamycin, ethyl methanesulfonate and
N-acetyl-2-acetoxyaminofluorene. These decreased SCE levels were accompanied by increased frequencies of chromosomal aberrations in the older cell populations. If SCE represents a form of DNA repair as has been suggested by several investigators, our findings would indicate impaired DNA repair occurring in old cells. |
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ISSN: | 0047-6374 1872-6216 |
DOI: | 10.1016/0047-6374(79)90107-6 |