Wild antelope skeletal muscle antioxidant enzyme activities do not correlate with muscle fibre type or oxidative metabolism

• Published in: Comparative biochemistry and physiology. Part A, Molecular & integrative physiology Vol. 242; p. 110638
• Main Authors: Hohl, Rodrigo, Blackhurst, Dee M., Donaldson, Byron, van Boom, Kathryn M., Kohn, Tertius A.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.04.2020
• Subjects: Animals; Antelopes - physiology; Antioxidants - metabolism; Citrate (si)-Synthase - metabolism; Creatine Kinase - metabolism; Glycolysis; L-Lactate Dehydrogenase - metabolism; Metabolism; Mitochondria; Muscle Fibers, Skeletal - metabolism; Muscle, Skeletal - enzymology; Myosin heavy chain; Oxidation-Reduction; Oxidative Stress; Superoxide Dismutase - metabolism; Wildlife; 3HAD; Oxidative stress; Myosin heavy chain; CK; SOD; MHC; GR; Metabolism; CS; LDH; Mitochondria; PFK; Wildlife
• ISSN: 1095-6433; 1531-4332
• DOI: 10.1016/j.cbpa.2019.110638

Wild antelope are some of the fastest land animals in the world, presenting with high oxidative and glycolytic skeletal muscle metabolism. However, no study has investigated their muscle antioxidant capacity, and may assist in understanding their physical ability and certain pathophysiological manifestations, such as capture myopathy. Therefore, the primary aim of this study was to determine the antioxidant activities superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR), as well as five key regulatory enzymes that serve as markers of glycolysis (phosphofructokinase (PFK) and lactate dehydrogenase (LDH)), the tricarboxylic acid cycle (citrate synthase (CS)), β-oxidation (3-hydroxyacetyl CoA dehydrogenase (3HAD)) and the phosphagen pathway (creatine kinase (CK)), in the Vastus lateralis muscle of six southern African wild antelope species (mountain reedbuck, springbok, blesbok, fallow deer, black wildebeest and kudu). Four different muscle groups from laboratory rats served as reference values for the enzyme activities. SOD, CS and LDH activities were the highest in the wild antelope, whereas CK appeared highest in rat fast twitch muscles. Between the wild antelope species, differences exist for SOD, CAT, PFK, CK and LDH, but not for CS, 3HAD and GR. CAT and GR correlated positively only with type I fibres. No correlations could be found between muscle fibre type and the oxidative enzymes, CS and 3HAD, from the wild animals, concurring with previous studies on porcine and rats. However, wild antelope and rat muscle CK and SOD strongly correlated, hinting towards an antioxidant role for CK. •Antioxidant capacity have not been studied in wild antelope species.•Vastus lateralis muscles from seven species were analysed.•Creatine kinase and superoxide dismutase correlated strongly.•Fibre type and oxidative capacity do not correlate with antioxidant capacity.