Aspirin modulates 2-hydroxyisobutyrylation of ENO1K281 to attenuate the glycolysis and proliferation of hepatoma cells

• Published in: Biochemical and biophysical research communications Vol. 560; pp. 172 - 178
• Main Authors: Yuan, Ying, Yuan, Hong-feng, Geng, Yu, Zhao, Li-na, Yun, Hao-lin, Wang, Yu-fei, Yang, Guang, Zhang, Xiao-dong
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 30.06.2021
• Subjects: 2-Hydroxyisobutyrylation; Aspirin; ENO1; Liver cancer; 2-Hydroxyisobutyrylation; Liver cancer; Aspirin; ENO1
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2021.04.083

Aspirin can efficiently inhibit the glycolysis and proliferation of cancer cells, however, the underlying mechanism is poorly understood. Here, we report that aspirin attenuates the glycolysis and proliferation of hepatoma cells through modulating the levels of lysine 2-hydroxyisobutyrylation (Khib) of enolase 1 (ENO1). We found that aspirin decreased the levels of glucose consumption and lactate production in hepatoma cells. Moreover, 4 mM aspirin reduced the activities of ENO1, a key enzyme of glycolysis, and decreased the levels of ENO1 Khib in the cells. Interestingly, we identified that 4 mM aspirin could decrease the levels of Khib on many proteins by using pan Khib antibody in the cells. Interestingly, the activities of ENO1 could be rescued by the transient overexpression of ENO1, but not by ENO1 mutant (K281R). Moreover, we identified that the C646, an inhibitor of p300 which is a writer of Khib, could reduce the levels of ENO1 Khib, resulting in the decrease of ENO1 activities. The treatment with PDTC, an inhibitor of NF-κB which is a target of aspirin, could work well as C646 in the cells. Both of aspirin and C646 (or PDTC) displayed a stronger effect than the single treatment in the system. Functionally, ENO1, but not ENO1 mutant (K281R), could rescue the aspirin-induced inhibition of proliferation of liver cancer cells in vitro, suggesting that ENO1K281 is involved in the aspirin-mediated inhibition of liver cancer. Our finding provides new insights into the mechanism by which aspirin attenuates the glycolysis and proliferation of hepatoma cells. •Aspirin decreases the glycolysis and activities of ENO1 in hepatoma cells.•Aspirin attenuates the levels of Khib of ENO1 in hepatoma cells.•ENO1K281 Khib is required for the decrease of glycolysis mediated by aspirin in hepatoma cells.•Aspirin-mediated ENO1K281 Khib confers the proliferation defective of hepatoma cells.