The methylation status in GNAS clusters May Be an epigenetic marker for oocyte quality

• Published in: Biochemical and biophysical research communications Vol. 533; no. 3; pp. 586 - 591
• Main Authors: Li, Qian-Nan, Li, Ang, Sun, Si-Min, Liu, Wen-Bo, Meng, Tie-Gang, Guo, Xing-Ping, Schatten, Heide, Sun, Qing-Yuan, Ou, Xiang-Hong
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 10.12.2020
• Subjects: Age Factors; Animals; Biomarkers; Cells, Cultured; CpG Islands; DNA Methylation; Female; Follicle culture; Genomic Imprinting; GNAS clusters; Mice; Oocyte; Oocytes - metabolism; Ovarian Follicle - cytology; DNA methylation; GNAS clusters; Oocyte; Follicle culture
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2020.09.055

During follicle growth, DNA methylation is gradually established, which is important for oocyte developmental competence. Due to the facts that oocytes from prepubertal individuals show reduced developmental outcomes when compared to those from sexually mature individuals, and the fact that oocytes derived from in vitro follicle culture have much lower developmental competence, it is worth exploring whether prepubertal superovulation and in vitro follicle culture will cause changes in DNA methylation imprinting status in oocytes. In this study, we found that the CpG island in maternally imprinted GNAS clusters was hypermethylated in the MII-stage oocytes from sexually mature mice, but was hypomethylated in oocytes from prepuberty individuals. The GNAS clusters in the MII-stage oocytes obtained by in vitro follicle culture showed heterogeneous methylation levels, indicating different qualities of oocytes, however, three other maternally imprinted genes, Peg1, Lot1 and Impact, were all hypermethylated in the MII-stage oocytes derived from both prepubertal superovulation and in vitro follicle culture. Taken together, the findings suggest that the methylation status in GNAS clusters may potentially represent a novel epigenetic marker for oocyte quality detection. •The methylation of CpG island of GNAS clusters in MII-stage oocytes is established in an age-dependent manner.•The methylation levels in GNAS clusters obtained by in vitro follicle culture fluctuated, showing different qualities of oocytes.•The methylation of GNAS clusters may potentially represent a novel epigenetic marker for oocyte quality detection.