Bacillus pocheonensis sp. nov., a moderately halotolerant, aerobic bacterium isolated from soil of a ginseng field

• Published in: International journal of systematic and evolutionary microbiology Vol. 57; no. 11; pp. 2532 - 2537
• Main Authors: Ten, Leonid N, Baek, Sang-Hun, Im, Wan-Taek, Larina, Liudmila L, Lee, Jung-Sook, Oh, Hee-Mock, Lee, Sung-Taik
• Format: Journal Article
• Language: English
• Published: England Soc General Microbiol 01.11.2007
• Subjects: Aerobiosis; Bacillus - chemistry; Bacillus - classification; Bacillus - genetics; Bacillus - isolation & purification; Bacillus fumarioli; Bacillus jeotgali; Bacillus pocheonensis; Bacterial Typing Techniques; DNA, Bacterial - analysis; DNA, Ribosomal - analysis; Fatty Acids - analysis; Genes, rRNA; Genotype; Korea; Molecular Sequence Data; Panax - growth & development; Phenotype; Phylogeny; Polymerase Chain Reaction - methods; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Sodium Chloride - metabolism; Soil Microbiology; Species Specificity; Korea
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.64491-0

1 Department of Biology and Medicinal Science, Pai Chai University, 14 Yeonja-1-Gil, Seo-Gu, Daejeon 302-735, Republic of Korea 2 Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1, Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea 3 Institute of Biochemical Physics, Kosigin st. 4, 119991 Moscow, Russia 4 Korean Collection for Type Cultures (KCTC), Korea Research Institute of Bioscience and Biotechnology, 52 Oeundong, Yusong-gu, Daejeon 305-333, Republic of Korea Correspondence Wan-Taek Im wandra{at}kaist.ac.kr A Gram-positive, non-motile, endospore-forming bacterial strain, designated Gsoil 420 T , was isolated from soil of a ginseng field in Pocheon Province, South Korea, and was characterized, using a polyphasic approach, in order to determine its taxonomic position. The novel isolate consisted of strictly aerobic, rod-shaped cells and was able to grow in medium supplemented with up to 12 % NaCl at 25 °C and pH 6.5–7.0. Comparative 16S rRNA gene sequence analysis showed that strain Gsoil 420 T fell within the radiation of the cluster comprising Bacillus species and formed a coherent cluster with Bacillus niacini (16S rRNA gene sequence similarity, 98.6 %), Bacillus bataviensis (98.6 %), Bacillus soli (98.3 %), Bacillus drentensis (98.0 %), Bacillus novalis (98.0 %), Bacillus vireti (97.9 %), Bacillus foraminis (97.6 %), Bacillus fumarioli (97.4 %) and Bacillus jeotgali (97.0 %). The levels of 16S rRNA gene sequence similarity with respect to other Bacillus species with validly published names were less than 96.8 %. Strain Gsoil 420 T had a genomic DNA G+C content of 44.9 mol% and the predominant respiratory quinone was MK-7. The major fatty acids were anteiso-C 15 : 0 (33.9 %), iso-C 15 : 0 (24.5 %) and iso-C 14 : 0 (19.9 %). These chemotaxonomic results supported the affiliation of strain Gsoil 420 T to the genus Bacillus. However, low DNA–DNA relatedness values and distinguishing phenotypic characteristics allowed genotypic and phenotypic differentiation of strain Gsoil 420 T from recognized Bacillus species. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain Gsoil 420 T represents a novel species of the genus Bacillus , for which the name Bacillus pocheonensis sp. nov. is proposed. The type strain is Gsoil 420 T (=KCTC 13943 T =DSM 18135 T ). These authors contributed equally to this work. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain Gsoil 420 T is AB245377 . An expanded neighbour-joining phylogenetic tree and DNA–DNA relatedness results for strain Gsoil 420 T and related Bacillus species are available as supplementary data with the online version of this paper.