The use of the Rx spin label in orientation measurement on proteins, by EPR

The bipedal spin label Rx is more restricted in its conformation and dynamics than its monopodal counterpart R1. To systematically investigate the utility of the Rx label, we have attempted to comprehensively survey the attachment of Rx to protein secondary structures. We have examined the formation...

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Bibliographic Details
Published inPhysical chemistry chemical physics : PCCP Vol. 18; no. 8; pp. 5799 - 5806
Main Authors Stevens, M A, McKay, J E, Robinson, J L S, El Mkami, H, Smith, G M, Norman, D G
Format Journal Article
LanguageEnglish
Published England 17.02.2016
Subjects
Attachment
Dynamics
Electron Spin Resonance Spectroscopy
Labels
Molecular Dynamics Simulation
Molecular structure
Orientation
Proteins
Proteins - chemistry
Spin Labels
Utilities
X-band
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Summary:The bipedal spin label Rx is more restricted in its conformation and dynamics than its monopodal counterpart R1. To systematically investigate the utility of the Rx label, we have attempted to comprehensively survey the attachment of Rx to protein secondary structures. We have examined the formation, structure and dynamics of the spin label in relation to the underlying protein in order to determine feasibility and optimum conditions for distance and orientation measurement by pulsed EPR. The labeled proteins have been studied using molecular dynamics, CW EPR, pulsed EPR distance measurement at X-band and orientation measurement at W-band. The utility of different modes and positions of attachment have been compared and contrasted.
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ISSN:1463-9076
1463-9084
DOI:10.1039/c5cp04753f