The modified G6PD deficiency screening test

• Published in: Accreditation and quality assurance Vol. 25; no. 2; pp. 121 - 126
• Main Authors: Krithong, Rungnapha, Nuinoon, Manit, Pramtong, Suputcha, Sasuk, Piyawit, Sarakul, Orawan
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.04.2020; Springer Nature B.V
• Subjects: Adenine; Analytical Chemistry; Anemia; Biochemistry; Chemistry; Chemistry and Materials Science; Color; Commercial Law; Dehydrogenases; Ecotoxicology; Enzymes; Erythrocytes; Fluorescence; Food Science; General Paper; Glucose; Glucose 6 phosphate dehydrogenase; Hemoglobin; Marketing; Nicotinamide; Nicotinamide adenine dinucleotide; Quantitative analysis; Reagents; Ultraviolet radiation; Milpitas California; United States > US; Jaundice; Hemolytic anemia; Screening test; Enzymopathy; G6PD deficiency; Fluorescent spot test
• ISSN: 0949-1775; 1432-0517
• DOI: 10.1007/s00769-019-01421-8

Glucose-6-phosphate dehydrogenase (G6PD) deficiency, which involves the mutation of the G6PD gene on the X chromosome, is the most common enzyme defect in humans. G6PD produces nicotinamide adenine dinucleotide phosphate (NADPH) for red blood cell protection from oxidative stress. Oxidative stress-induced red blood cell hemolysis in G6PD-deficient patients leads to anemia, jaundice, acute renal failure, and kernicterus in newborns. Currently, the fluorescent spot test (FST) is widely used for G6PD deficiency screening. This test detects NADPH production under UV light. This study purposed to modify the G6PD deficiency screening test based on NADPH production and the ability to detect the results of the color change in the color of the reaction. Blood samples of 60 subjects were collected and screened for G6PD deficiency using FST method. All samples were tested with the modified G6PD screening test and the quantitative assay kit. The results from the modified test showed the color red for normal cases, whereas the color brown indicated G6PD deficiency cases. The cutoff value of G6PD activity detected by the quantitative method was less than 2.9 U/gHb for G6PD deficiency. The precision of this study was 100 %. The results of sensitivity, specificity, negative predictive value (NPV), and positive predictive value (PPV) of the modified test were 100 %, 96.7 %, 100 %, and 96.7 %, respectively. The modified G6PD screening test can be used for the screening of G6PD deficiency in newborns, for support through providing more information, and for self-protection from oxidative stress.