Fluorometric measurement of DNA reassociation kinetics

A simple, highly sensitive method for assessment of the kinetics of reassociation of DNA has been developed. The method is based on the difference between the intensity of fluorescence of the ethidium bromide complex with single- and double-stranded DNA and requires no preliminary preparative separa...

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Published inAnalytical biochemistry Vol. 94; no. 1; pp. 121 - 124
Main Authors Hanson, K.P., Zvonareva, N.B., Ostashevsky, Y.Ya, Zhivotovsky, B.D.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.04.1979
Subjects
Animals
Chemical Phenomena
Chemistry
DNA - analysis
DNA, Single-Stranded - analysis
Ethidium
Kinetics
Rats
Spectrometry, Fluorescence
Thymus Gland
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Abstract A simple, highly sensitive method for assessment of the kinetics of reassociation of DNA has been developed. The method is based on the difference between the intensity of fluorescence of the ethidium bromide complex with single- and double-stranded DNA and requires no preliminary preparative separation of the molecules.
AbstractList A simple, highly sensitive method for assessment of the kinetics of reassociation of DNA has been developed. The method is based on the difference between the intensity of fluorescence of the ethidium bromide complex with single- and double-stranded DNA and requires no preliminary preparative separation of the molecules.
Author Ostashevsky, Y.Ya
Zvonareva, N.B.
Zhivotovsky, B.D.
Hanson, K.P.
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10.1126/science.161.3841.529
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Britten, Kohne (BIB1) 1968; 161
Hanson, Zhivotovsky (BIB6) 1975; 28
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LePecq, Paoletti (BIB3) 1966; 17
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Hanson, Zhivotovsky (BIB5) 1974; 78
Paetkau, Langman (BIB2) 1975; 65
LePecq (10.1016/0003-2697(79)90801-7_BIB3) 1966; 17
LePecq (10.1016/0003-2697(79)90801-7_BIB4) 1967; 27
Britten (10.1016/0003-2697(79)90801-7_BIB1) 1968; 161
Mazin (10.1016/0003-2697(79)90801-7_BIB7) 1974; 61
Wetmur (10.1016/0003-2697(79)90801-7_BIB9) 1968; 31
Hanson (10.1016/0003-2697(79)90801-7_BIB6) 1975; 28
Paetkau (10.1016/0003-2697(79)90801-7_BIB2) 1975; 65
Britten (10.1016/0003-2697(79)90801-7_BIB8) 1974; Vol. 29
Hanson (10.1016/0003-2697(79)90801-7_BIB5) 1974; 78
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Snippet A simple, highly sensitive method for assessment of the kinetics of reassociation of DNA has been developed. The method is based on the difference between the...
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SubjectTerms Animals
Chemical Phenomena
Chemistry
DNA - analysis
DNA, Single-Stranded - analysis
Ethidium
Kinetics
Rats
Spectrometry, Fluorescence
Thymus Gland
Title Fluorometric measurement of DNA reassociation kinetics
URI https://dx.doi.org/10.1016/0003-2697(79)90801-7
https://www.ncbi.nlm.nih.gov/pubmed/464276
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