Fluorometric measurement of DNA reassociation kinetics
A simple, highly sensitive method for assessment of the kinetics of reassociation of DNA has been developed. The method is based on the difference between the intensity of fluorescence of the ethidium bromide complex with single- and double-stranded DNA and requires no preliminary preparative separa...
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Published in | Analytical biochemistry Vol. 94; no. 1; pp. 121 - 124 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
United States
Elsevier Inc
01.04.1979
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Subjects | |
Online Access | Get full text |
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Summary: | A simple, highly sensitive method for assessment of the kinetics of reassociation of DNA has been developed. The method is based on the difference between the intensity of fluorescence of the ethidium bromide complex with single- and double-stranded DNA and requires no preliminary preparative separation of the molecules. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0003-2697 1096-0309 |
DOI: | 10.1016/0003-2697(79)90801-7 |