Mir-34c affects the proliferation and pluripotency of porcine induced pluripotent stem cell (piPSC)-like cells by targeting c-Myc

• Published in: Cells & development Vol. 166; p. 203665
• Main Authors: Yue, Wei, Sun, Jing, Zhang, Juqing, Chang, Yongxing, Shen, Qiaoyan, Zhu, Zhenshuo, Yu, Shuai, Wu, Xiaolong, Peng, Sha, Li, Na, Hua, Jinlian
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.06.2021
• Subjects: c-Myc; Cell proliferation; miR-34c; piPSC-like cells; Pluripotency; Cell proliferation; c-Myc; Pluripotency; miR-34c; piPSC-like cells
• ISSN: 2667-2901; 2667-2901
• DOI: 10.1016/j.cdev.2021.203665

MicroRNAs are important regulators in stem cells, which involve in gene regulation, including cell proliferation, differentiation and apoptosis. As an important one, miR-34c participates in various processes by targeting protein-coding genes. It is generally considered as a tumor suppressor and cell adhesion inhibitor. However, whether miR-34c has effects on pluripotent stem cells is not clear. Here, by mir-34c mimics transfection, the function of miR-34c on porcine induced pluripotent stem cell (piPSC)-like cells was investigated. Bioinformatics analyses showed that c-Myc is miR-34c's candidate target, which was confirmed by dual Luciferase assay. The knockout of miR-34c indicated that mir-34c affects the proliferation and pluripotency of piPSC-like cells by targeting c-Myc. Our study explored the regulatory mechanism of miR-34c on piPSC-like cells, providing a reference for the establishment of true porcine PSCs. •C-Myc is miR-34c’s putative target.•Mir-34c affects the proliferation and pluripotency of piPS-like cells by targeting c-Myc.•Mir-34c inhibits the proliferation of piPS-like cells and reduces the pluripotency of piPS-like cells.