Anti-TNF treatment corrects IFN-γ–dependent proinflammatory signatures in Blau syndrome patient–derived macrophages

• Published in: Journal of allergy and clinical immunology Vol. 149; no. 1; pp. 176 - 188.e7
• Main Authors: Kitagawa, Yohko, Kawasaki, Yuri, Yamasaki, Yuichi, Kambe, Naotomo, Takei, Syuji, Saito, Megumu K.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.01.2022
• Subjects: Adult; anti-TNF treatment; Arthritis - drug therapy; Arthritis - genetics; Arthritis - immunology; autoinflammatory loop; Blau syndrome; Cell Line; Child; Child, Preschool; disease-specific iPS cells; Female; Humans; Induced Pluripotent Stem Cells - cytology; Interferon-gamma - immunology; Macrophages - immunology; Male; NF-kappa B - immunology; NF-κB; Sarcoidosis - drug therapy; Sarcoidosis - genetics; Sarcoidosis - immunology; Synovitis - drug therapy; Synovitis - genetics; Synovitis - immunology; Transcriptome; Tumor Necrosis Factor Inhibitors - pharmacology; Tumor Necrosis Factor Inhibitors - therapeutic use; Uveitis - drug therapy; Uveitis - genetics; Uveitis - immunology; Young Adult; disease-specific iPS cells; M-CSF; iPSC; GO; NOD2; MAPK; PBMC; PCA; VEGFA; NF-κB; BS; Blau syndrome; autoinflammatory loop; MDP; KEGG; WT; anti-TNF treatment
• ISSN: 0091-6749; 1097-6825
• DOI: 10.1016/j.jaci.2021.05.030

Blau syndrome (BS) is an autoinflammatory disease associated with mutations in nucleotide-binding oligomerization domain 2. Although treatments with anti-TNF agents have been reported to be effective, the underlying molecular mechanisms remain unclear. We aimed to elucidate the mechanisms of autoinflammation in patients with BS and to clarify how anti-TNF treatment controls the disease phenotype at the cellular level in clinical samples. Macrophages were differentiated from monocytes of 7 BS patients, and global transcriptional profiles of 5 patients were analyzed with or without IFN-γ stimulation. Macrophages were also generated from BS-specific induced pluripotent stem cells (iPSCs), and their transcriptome was examined for comparison. Aberrant inflammatory responses were observed upon IFN-γ stimulation in macrophages from untreated BS patients, but not in those from patients treated with anti-TNF. iPSC-derived macrophages carrying a disease-associated mutation also showed IFN-γ–dependent accelerated inflammatory responses. Comparisons of peripheral blood– and iPSC-derived macrophages revealed the upregulation of nuclear factor kappa–light-chain enhancer of activated B cells (NF-κB) targets in unstimulated macrophages as a common feature. IFN-γ stimulation is one of the key signals driving aberrant inflammatory responses in BS-associated macrophages. However, long-term treatment with anti-TNF agents ameliorates such abnormalities even in the presence of IFN-γ stimulation. Our data thus suggest that preexposure to TNF or functionally similar cytokines inducing NF-κB–driven proinflammatory signaling during macrophage development is a prerequisite for accelerated inflammatory responses upon IFN-γ stimulation in BS.