Ontogeny of immunoglobulin expression in the Mexican axolotl

• Published in: Development (Cambridge) Vol. 107; no. 2; pp. 253 - 263
• Main Authors: Fellah, J S, Vaulot, D, Tournefier, A, Charlemagne, J
• Format: Journal Article
• Language: English
• Published: England The Company of Biologists Limited 01.10.1989
• Subjects: Age Factors; Ambystoma - growth & development; Ambystoma mexicanum; Ambystoma mexicanum - genetics; Ambystoma mexicanum - growth & development; Ambystoma mexicanum - immunology; Animals; Blotting, Western; Enzyme-Linked Immunosorbent Assay; Fluorescent Antibody Technique; Gastric Mucosa - metabolism; Gene Expression; Immunoglobulins - biosynthesis; Intestinal Mucosa - metabolism; Liver - metabolism; Lymphocytes - cytology; Lymphocytes - immunology; Mesonephros - metabolism; Microscopy, Phase-Contrast; Spleen - embryology; Spleen - immunology
• ISSN: 0950-1991; 1477-9129
• DOI: 10.1242/dev.107.2.253

The ontogeny of immunoglobulin (Ig) synthesis was followed at both cellular and serological levels in the Mexican axolotl (Ambystoma mexicanum) using polyclonal antibodies recognizing all Ig molecules and a set of monoclonal antibodies (Mabs) specific for the C mu and Cv heavy Ig chain isotypes and for the light chain constituents shared by IgM and IgY molecules. Clusters of IgM- and of IgY-synthesizing lymphocytes, often located in separate sites, are first present in spleen sections of 7-week-old 25 mm larvae, about one month after differentiation of the spleen anlage (stage 39–40). In 12-week-old 30–35 mm larvae, the relative proportion of IgM- and IgY-synthesizing cells in the spleen is the same as that in adult animals. However, a marked enhancement of the spleen B cell compartment occurs from 5 to 9 months when Ig-positive cells represent about 88% of the lymphocytes population compared to 60% in adults. No structures equivalent to B cell germinal centers were observed at any stage of the spleen differentiation and cells, although often clustered in small groups, remain dispersed in the entire organ. The relative proportions of IgM and IgY B cells throughout the spleen remain constant during development (about 1 IgY+ cell for 5–6 IgM+ cells) and IgM molecules are first detected in the serum of 2.5-month-old larvae. The enhancement of the serum IgM level correlates well with the absolute number of IgM+ cells in the growing spleen. IgY molecules cannot be detected in the serum before the 7th month but their level quickly increases to reach about 60% of the adult value at 10 months. Thyroxine-induced metamorphosis or hyperimmunization of 4- to 6-month-old larvae had no effect upon the temporal expression of the Ig classes in serum.