Biosynthesis of triacylglycerols in the intestines of rainbow trout ( salmo gairdnerii) fed marine zooplankton rich in wax esters

• Published in: Biochimica et biophysica acta Vol. 575; no. 3; pp. 358 - 364
• Main Authors: Bauermeister, Anne E.M., Sargent, John R.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 18.12.1979
• Subjects: Animals; Diet; Esters; Intestinal Mucosa - metabolism; Plankton; Rainbow trout intestine; Salmonidae - metabolism; Triacylglycerol biosynthesis; Triglycerides - biosynthesis; Trout - metabolism; Wax ester diet, Zooplankton; Waxes; Rainbow trout intestine; HEPES; Triacylglycerol biosynthesis; Wax ester diet, Zooplankton
• ISSN: 0005-2760; 0006-3002; 1879-145X
• DOI: 10.1016/0005-2760(79)90104-8

Intestinal preparations from rainbow trout fed a diet rich in wax esters incorporated [1- 14C]hexadecanoic acid and [1- 14C]hexadecanol into triacylglycerols at the same rate. The ratio of the number of H atoms from C 1 of hexadecanol to the number of molecules of hexadecanol incorporated into triacylglycerols was 1.6: 3.0. [U- 14C]Glucose was incorporated much faster into the glycerol moiety of triacylglycerols than was [U- 14C]aspartic acid. We conclude that the oxidation of absorbed fatty alcohol to fatty acid and its subsequent incorporation into triacylglycerols is closely linked with the reductive formation of triacylglycerol-glycerol from glucose. The ability of trout intestines to metabolise fatty alcohol to triacylglycerols was the same in fish fed wax esters as in those fed triacylglycerols.