Dynamic alterations and durability of T helper 22 and its corresponding cytokines following treatment in pediatric visceral leishmaniasis
[Display omitted] •Th22 was more frequent in VL patients than controls.•Th22 significantly down-regulated in patients upon treatment.•IL-6 and IL-21 were significantly higher in VL patients’ sera than controls.•Th22 corresponding cytokines altered in patients’ sera after treatment.•Th22 and the corr...
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Published in | Cytokine (Philadelphia, Pa.) Vol. 144; p. 155579 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
Elsevier Ltd
01.08.2021
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Subjects | |
Online Access | Get full text |
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Summary: | [Display omitted]
•Th22 was more frequent in VL patients than controls.•Th22 significantly down-regulated in patients upon treatment.•IL-6 and IL-21 were significantly higher in VL patients’ sera than controls.•Th22 corresponding cytokines altered in patients’ sera after treatment.•Th22 and the corresponding cytokines may play a role in VL pathogenesis.
Considering the collaboration between immune responses and medications for the improvement of visceral leishmaniasis (VL), this study investigated the levels of T helper (Th) 22 and the corresponding cytokines in the acute phase of VL and their alterations following treatment.
The study was conducted on 18 patients with confirmed VL and 20 healthy sex and age matched children as the controls. The levels of Th22 cells and the cytokines driving their differentiations and functions in the blood and peripheral blood mononuclear cells (PBMC) cultured supernatants, were assessed using flow cytometry method. The results revealed significantly higher levels of Th22, IL-21 and IL-6 in the patients’ blood than those in the controls. Additionally, higher levels of IL-21 and IL-22 were observed in the cultured supernatants of VL patients’ PBMCs, compared to the controls. Upon treatment, Th22 and IL-6 were down-regulated and conversely, IL-21, IL-22, IL-23 and IL-33 were significantly up-regulated in the patients’ blood at different time points. Receiver operating characteristic (ROC) curve analysis indicated that for the differential diagnosis of VL, plasma IL-21 is more sensitive and specific than Th22 and the above mentioned cytokines.
The higher proportions of Th22 and IL-21 in the VL patients and their alterations post treatment confer their roles in the immunopathogenesis of VL. So, Th22 and IL-21 in the patients’ blood can be considered as biomarkers to be used for the differential diagnosis of VL. Nevertheless, further studies are warranted to clarify their particular mechanisms in this process. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1043-4666 1096-0023 |
DOI: | 10.1016/j.cyto.2021.155579 |