Characterisation of mammary tumour virus of strain ICRC mouse
The mouse mammary tumour virus (MuMTV) isolated and purified from the milk of strain ICRC mice, susceptible to spontaneous development of mammary cancer and leukemia, was investigated for its morphological, biophysical, immunological and structural characters. The purified virions banded at the dens...
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Published in | European journal of cancer Vol. 14; no. 3; pp. 251 - 261 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
England
Elsevier Ltd
01.03.1978
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Subjects | |
Online Access | Get full text |
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Summary: | The mouse mammary tumour virus (MuMTV) isolated and purified from the milk of strain ICRC mice, susceptible to spontaneous development of mammary cancer and leukemia, was investigated for its morphological, biophysical, immunological and structural characters.
The purified virions banded at the density of
1.17 g/ml in
0–55% linear sucrose gradient and contain mature B type particles along with few C type particles when studied under the electron microscope. The polyacrylamide gel electrophoresis of strain ICRC MuMTV reproducibly resolved at least ten polypeptides. The mol. wt of the three major polypeptides are
49,000 (p
49), 34,000 (p
34) and
24,000 (p
24). Two of these polypeptides p
49 and p
34 have been characterised as glycoproteins on the basis of staining with periodic acid Schiff's reagent. High titers of MuMTV antigens were noted in the milk plasma of strain ICRC mice. The anti MTV-S serum could detect common antigens in disrupted virions of strains ICRC and C
3H(Jax) by immunodiffusion test.
Strain ICRC was a high cancer strain in the earlier generations of inbreeding (the average tumour incidence in the breeders of first ten generations was
81.3%) however, the tumour incidence in the
45th generation has gone down considerably. The parameters studied in the present investigations have indicated that the MuMTV of strain ICRC exhibit characters comparable to those of high cancer strain C
3H(Jax), in spite of a significant decrease in the mammary tumour incidence. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0014-2964 |
DOI: | 10.1016/0014-2964(78)90188-3 |