Proposal for a common oligosaccharide intermediate in the synthesis of membrane glycoproteins

• Published in: Cell Vol. 12; no. 4; pp. 893 - 900
• Main Authors: Robbins, Phillips W., Hubbard, S.Catherine, Turco, S.J., Wirth, Dyann F.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.12.1977
• Subjects: Acetylglucosaminidase - metabolism; Asparagine - metabolism; Glycopeptides - metabolism; Glycoproteins - biosynthesis; Lipid Metabolism; Mannose - metabolism; Membrane Proteins - biosynthesis; Sindbis Virus; Vesicular stomatitis Indiana virus; Viral Proteins - biosynthesis

Endo-β-N-acetylglucosaminidase H (endo H) is an enzyme which acts on asparagine- and lipid-linked oligosaccharides containing five or more mannose residues. Complex oligosaccharides and glycopeptides are completely resistant to the action of the enzyme. We have carried out pulse-chase experiments wi...