Proposal for a common oligosaccharide intermediate in the synthesis of membrane glycoproteins

• Published in: Cell Vol. 12; no. 4; pp. 893 - 900
• Main Authors: Robbins, Phillips W., Hubbard, S.Catherine, Turco, S.J., Wirth, Dyann F.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.12.1977
• Subjects: Acetylglucosaminidase - metabolism; Asparagine - metabolism; Glycopeptides - metabolism; Glycoproteins - biosynthesis; Lipid Metabolism; Mannose - metabolism; Membrane Proteins - biosynthesis; Sindbis Virus; Vesicular stomatitis Indiana virus; Viral Proteins - biosynthesis
• ISSN: 0092-8674; 1097-4172
• DOI: 10.1016/0092-8674(77)90153-2

Endo-β-N-acetylglucosaminidase H (endo H) is an enzyme which acts on asparagine- and lipid-linked oligosaccharides containing five or more mannose residues. Complex oligosaccharides and glycopeptides are completely resistant to the action of the enzyme. We have carried out pulse-chase experiments with 35S-methionine and 3H-mannose in uninfected cells and in cells infected with Sindbis virus and vesicular stomatitis virus (VSV). In each case, the labeled materials were analyzed for sensitivity to endo H by polyacrylamide gel electrophoresis and gel filtration. We find that endo H releases all the labeled mannose from pulse-labeled proteins. Initially, the released material is nearly identical in size to the endo H cleavage product derived from lipid-linked oligosaccharides present in the same cells. During chase periods, 35S-methionine and 3H-mannose protein becomes increasingly resistant to the enzyme. Moreover, the 3H-mannose-labeled material released from the protein during chase periods is smaller in size than the oligosaccharide from the lipid. On the basis of these results and results from other laboratories, we propose that during glycosylation of asparagine residues, a common oligosaccharide is transferred from the lipid carrier to protein and is subsequently processed to yield the so-called “high mannose” and “complex” oligosaccharides. Since, on the basis of present evidence, the lipid-linked oligosaccharide contains two N-acetylglucosamine, 8–12 mannose and 1–2 glucose molecules, it seems probable that the carbohydrate-processing systems remove half or more of the mannose and all of the glucose residues at sites destined to become complex glycopeptides. Removal of mannose and glucose residues may also occur at sites destined to become mature high mannose glycopeptides.