Agrobacterium tumefaciens -mediated genetic transformation of the white root rot ascomycete Rosellinia necatrix

Hygromycin B resistance was conferred to the mycelium of the white root rot fungus Rosellinia necatrix by transformation with the hygromycin B phosphotransferase gene (hph) of Escherichia coli under the control of the heterologous fungal Aspergillus nidulans P-gpd (glyceraldehyde 3-phosphate dehydro...

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Bibliographic Details
Published inMycoscience Vol. 46; no. 1; pp. 27 - 31
Main Authors Aimi, Tadanori, Kitamoto, Yutaka, Taguchi, Hiroyuki, Tanaka, Yutaka, Morinaga, Tsutomu
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.02.2005
Subjects
AGROBACTERIUM TUMEFACIENS
Ascomycetes
ASCOMYCOTINA
Aspergillus nidulans
GENE TRANSFER
GENETIC ENGINEERING
GENETIC TRANSFORMATION
GENETICALLY MODIFIED MICROORGANISMS
Hygromycin B
ROOTS
ROSELLINIA
Rosellinia necatrix
ROTS
Transformation
Agrobacterium tumefaciens
Rosellinia necatrix
Transformation
Hygromycin B
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Summary:Hygromycin B resistance was conferred to the mycelium of the white root rot fungus Rosellinia necatrix by transformation with the hygromycin B phosphotransferase gene (hph) of Escherichia coli under the control of the heterologous fungal Aspergillus nidulans P-gpd (glyceraldehyde 3-phosphate dehydrogenase) promoter and the trpC terminator. In all three transformants, the presence of hph and single-copy integrations of the marker gene were demonstrated by Southern analysis. This is the first report describing A. tumefaciens-mediated transformation of R. necatrix
Bibliography:F30
H20
2006001373
ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:1340-3540
1618-2545
DOI:10.1007/S10267-004-0210-Z