Hypolipidemic constituents from the aerial portion of Sibiraea angustata

• Published in: Bioorganic & medicinal chemistry letters Vol. 30; no. 11; p. 127161
• Main Authors: Liu, Hongdong, Zhang, Ting, Jiang, Piao, Zhu, Weifeng, Yu, Songhua, Liu, Yong, Li, Bin, Li, Fei
• Format: Journal Article
• Language: English
• Published: OXFORD Elsevier Ltd 01.06.2020; Elsevier
• Subjects: Chemistry; Chemistry, Medicinal; Chemistry, Organic; Fatty Acid Synthases - antagonists & inhibitors; Fatty Acid Synthases - metabolism; Glycosides - chemistry; Glycosides - isolation & purification; Hep G2 Cells; Humans; Hypolipidemic effect; Life Sciences & Biomedicine; Lipid Metabolism - drug effects; Lipidomics; Magnetic Resonance Spectroscopy; Molecular Conformation; Monoterpene acylglucoside; Monoterpenes - chemistry; Monoterpenes - isolation & purification; Oleic Acid - pharmacology; Pharmacology & Pharmacy; Phosphatidylcholines - metabolism; Physical Sciences; Plant Components, Aerial - chemistry; Plant Components, Aerial - metabolism; Plant Extracts - chemistry; Plant Extracts - pharmacology; Principal Component Analysis; Rosaceae - chemistry; Rosaceae - metabolism; Science & Technology; Sibiraea angustata; Triglycerides - metabolism; Lipidomics; Sibiraea angustata; Hypolipidemic effect; Monoterpene acylglucoside; MONOTERPENE GLUCOSIDES; MODULATION
• ISSN: 0960-894X; 1464-3405
• DOI: 10.1016/j.bmcl.2020.127161

[Display omitted] Two new monoterpene acylglucosides (1–2) and one new aromatic glycoside (3), together with five known compounds (4–8), were isolated from 95% ethanol extract of Sibiraea angustata. The structures of these compounds were characterized by 2D-NMR and mass spectrometry. Compounds were evaluated for their hypolipidemic activity using oleic acid-induced lipid accumulation in HepG2 cells. RT-PCR analysis revealed that compound 5 could decrease the expression level of fatty acid synthase (FASN). Lipidomics analysis indicated that compound 5 significantly decreased the levels of 11 lipids in oleic acid-induced lipid accumulation, including triglycerides (TG), diglycerides (DG), phosphatidylcholines (PC) and 1-acyl-sn-glycero-3-phosphocholines (lysoPC). These data demonstrated that terpene acylglucosides are the major active constituents in Sibiraea angustata.