Down-regulation of Coxsakie and Adenovirus Receptor during Embryo Implantation

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 32; no. 1; pp. 103 - 106
• Main Author: 李豫峰 赵焕 王蓓蓓 崔丹丹 袁素珍 贺晓 郭娜 陈宁宁
• Format: Journal Article
• Language: English
• Published: Heidelberg Huazhong University of Science and Technology 01.02.2012
• Subjects: Adenovirus; and; Animals; Cavities; Computer programs; coxsackie; Coxsackie and Adenovirus Receptor-Like Membrane Protein - metabolism; Down-Regulation - physiology; Embryo Implantation - physiology; Embryos; Epithelium; Female; Gene expression; Immunohistochemistry; implantation;endometrium; Medicine; Medicine & Public Health; Mice; mRNA; Polymerase chain reaction; receptor;embryo; Sexual intercourse; software; Tight junctions; Tissue Distribution; Uterus - metabolism; endometrium; coxsackie and adenovirus receptor; embryo implantation
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-012-0018-6

In this study,real-time PCR and immunohistochemistry were used to detect coxsakie and adenovirus receptor (CAR) expression.Both localization and quantity were evaluated in the uteri ob-tained at days post coitus (dpc) 2.5,4.5,6.5,8.5.Outcome of PCR was assessed by 2-ΔΔCt method.Im-age Pro-Plus 6.0 software was used for quantifying mean density of CAR expression in immunohisto-chemical sections.We found relatively weak CAR expression in the mouse uteri during implantation window.PCR and immunohistochemistry revealed highest CAR expression was detected on dpc 2.5 followed by down-regulation of CAR at dpc 4.5 and 6.5 (with significant difference).At dpc 8.5,CAR expression was increased slightly again.It is concluded that during implantation,the expression of CAR mRNA and protein is declined,resulting in the impairment of tight junction between cavity epithelium cells.After implantation window closure,CAR appears again to maintain epithelium stability.CAR might play an important role during embryo implantation procedure.