Gold nanoparticles coupled with graphene quantum dots in organized medium to quantify aminoglycoside anti-biotics in yellow fever vaccine after solid phase extraction using a selective imprinted polymer

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 158; pp. 480 - 493
• Main Authors: Toloza, Carlos A.T., Almeida, Joseany M.S., Khan, Sarzamin, dos Santos, Yasmin G., da Silva, Andrea R., Romani, Eric C., Larrude, Dunieskys G., Freire, Fernando L., Aucélio, Ricardo Q.
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 05.09.2018
• Subjects: Amino-functionalized graphene quantum dots; Gold nanoparticles; Kanamycin sulfate; Organized medium; Photoluminescence on/off; Organized medium; Photoluminescence on/off; Amino-functionalized graphene quantum dots; Gold nanoparticles; Kanamycin sulfate
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/j.jpba.2018.05.050

[Display omitted] •AuNPs were synthesized on a dispersion of GQDs-amino using NaBH4 as reducing agent.•Reaction was made in organized solution containing the cationic surfactant CTAB.•Kanamycin amplify photoluminescence from the AuNPs-GQDs-amino-CTAB probe.•The instrumental detection limit was 30 nmol L−1.•Solid phase extraction using an aminoglycoside selective MIP ensured selectivity.•Kanamycin was detected in pharmaceutical formulation and in yellow-fever vaccine. The determination of kanamycin sulfate was made indirectly by measuring its effect on photoluminescent amino functionalized graphene quantum dots (GQDs-amino) associated with gold nanoparticles (AuNPs) that were produced by the reduction of AuCl4 with NaBH4 in an aqueous dispersion of GQDs-amino (obtained by the pyrolysis of citric acid and glutathione) also containing the cationic surfactant CTAB. The AuNPs-GQDs-amino-CTAB system presents a suppressed photoluminescence that is amplified in the presence of kanamycin. Under optimized experimental conditions, the photoluminescence amplification of the nanomaterial system showed a linear response as a function of kanamycin concentration, covering three orders of magnitude (10−7 to 10−5 mol L−1). The use of solid phase extraction with a cartridge packed with aminoglycoside selective molecularly imprinted polymer ensured selectivity in determinations made on yellow-fever vaccine and veterinary pharmaceutical formulations. The analytical results were statistically similar to those obtained with an HPLC-based fluorescence method (after chemical derivatization). The proposed method is a simple, sensitive and selective approach that does not involve the use of toxic reagents employed for chemical derivatization of aminoglycoside antibiotics.