A branching stochastic evolutionary model of the B-cell repertoire

• Published in: Journal of mathematical biology Vol. 89; no. 1; p. 10
• Main Authors: Hyrien, Ollivier, Yanev, Nikolay M.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.07.2024; Springer Nature B.V
• Subjects: Animals; Applications of Mathematics; B-Lymphocytes - immunology; Branching; Branching (mathematics); Diversity indices; Evolution; Germinal Center - cytology; Germinal Center - immunology; Germinal centers; Humans; Immigration; Immunization; Light chains; Lymphocytes B; Mathematical and Computational Biology; Mathematical Concepts; Mathematics; Mathematics and Statistics; Models, Immunological; Nucleotide sequence; Nucleotides; Receptor mechanisms; Receptors, Antigen, B-Cell - genetics; Receptors, Antigen, B-Cell - immunology; Somatic Hypermutation, Immunoglobulin - genetics; Stochastic Processes; Stochasticity; Multitype branching process; 60J85; Temporal alpha and beta diversity; Sequential immunization; Germinal center; 60J80; Convergent evolution
• ISSN: 0303-6812; 1432-1416; 1432-1416
• DOI: 10.1007/s00285-024-02102-y

We propose a stochastic framework to describe the evolution of the B-cell repertoire during germinal center (GC) reactions. Our model is formulated as a multitype age-dependent branching process with time-varying immigration. The immigration process captures the mechanism by which founder B cells initiate clones by gradually seeding GC over time, while the branching process describes the temporal evolution of the composition of these clones. The model assigns a type to each cell to represent attributes of interest. Examples of attributes include the binding affinity class of the B cells, their clonal family, or the nucleotide sequence of the heavy and light chains of their receptors. The process is generally non-Markovian. We present its properties, including as t → ∞ when the process is supercritical, the most relevant case to study expansion of GC B cells. We introduce temporal alpha and beta diversity indices for multitype branching processes. We focus on the dynamics of clonal dominance, highlighting its non-stationarity, and the accumulation of somatic hypermutations in the context of sequential immunization. We evaluate the impact of the ongoing seeding of GC by founder B cells on the dynamics of the B-cell repertoire, and quantify the effect of precursor frequency and antigen availability on the timing of GC entry. An application of the model illustrates how it may help with interpretation of BCR sequencing data.