GUS-reporter based analysis of the promoter activity of Gossypium hirsutum NAC transcription factor, GhNAC4 that is induced by phytohormones and environmental stresses
The expression analysis of GhNAC4 , a NAC ( NAM, ATAF1 - 2 , and CUC2 ) domain-containing transcription factor of the upland cotton, Gossypium hirsutum was carried out. Its expression was up-regulated by abscisic acid, cytokinin, methyl jasmonic acid, gibberellic acid, auxin, and ethylene. Its expre...
Saved in:
Published in | Plant cell, tissue and organ culture Vol. 141; no. 3; pp. 643 - 654 |
---|---|
Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Dordrecht
Springer Netherlands
01.06.2020
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | The expression analysis of
GhNAC4
, a NAC (
NAM, ATAF1
-
2
, and
CUC2
) domain-containing transcription factor of the upland cotton,
Gossypium hirsutum
was carried out. Its expression was up-regulated by abscisic acid, cytokinin, methyl jasmonic acid, gibberellic acid, auxin, and ethylene. Its expression was also highly induced by drought, osmotic, oxidative, salinity, high and low-temperature stresses, and wounding. To corroborate these observations, we cloned the promoter of
GhNAC4
and fused it transcriptionally with
uidA
(GUS) gene for studies in transgenic tobacco. Fluorometric GUS analysis of the transgenic plants revealed that it is also induced by various phytohormones and environmental stresses. The spatio-temporal analysis of the
GhNAC4
promoter revealed that GUS expression was active in all stages of plant development including the reproductive organs. In the mature plant, the
GhNAC4
is expressed at a higher level in vascular bundles and guard cells. We also observed intense expression in other cells upon wounding. These observations were supported by a detailed bioinformatic analysis of the
GhNAC4
promoter for identifying the
cis
-acting elements that are associated with the regulation of gene expression in a tissue-specific and induced manner.
Key message
GhNAC4
gene is induced by phytohormones and environmental stress treatments in cotton. It is expressed throughout a mature plant, including the reproductive organs, vascular bundles, and guard cells. |
---|---|
ISSN: | 0167-6857 1573-5044 |
DOI: | 10.1007/s11240-020-01825-2 |